文献类型：期刊文献

英文题名：A PET-based fluorescent probe for monitoring labile Fe(II) pools in macrophage activations and ferroptosis

作者：Xing, Wanjin[1,2];Xu, Hang[1,2];Ma, Huijuan[1,2];Abedi, Syed Ali Abbas[3];Wang, Shanshan[1,2];Zhang, Xingchen[1,2];Liu, Xiaogang[3];Xu, Huan[1,2];Wang, Wei[4,5];Lou, Kaiyan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioengn Reactor, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Me, Sch Pharm,Shanghai Key Lab New Drug Design, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Singapore Univ Technol & Design, Fluorescence Res Grp, 8 Somapah Rd, Singapore 487372, Singapore;[4]Univ Arizona, Dept Pharmacol & Toxicol, Tucson, AZ 85721 USA;[5]Univ Arizona, BIO5 Inst, Tucson, AZ 85721 USA

年份：2022

卷号：58

期号：18

起止页码：2979

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20221111774913);WOS:【SCI-EXPANDED(收录号:WOS:000753964100001)】；

基金：The financial support by Shanghai Natural Science Fund (Grant No. 20ZR1414700), Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28), Shanghai Sailing Program (Grant No. 19YF1412500), the National Natural Science Foundation of China (Grant No. 21577037, 21738002, 21906057), and the State Key Laboratory of Bioreactor Engineering, and the Ministry of Education, Singapore (MOE-MOET2EP10120-0007), is greatly appreciated.

语种：英文

外文关键词：Fluorophores - Macrophages - Fluorescence spectroscopy - Iron compounds - Chemical activation - Fluorescence quenching

摘要：A fluorescent probe (COU-LIP-1) for monitoring labile Fe(II) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(II)-induced reductive cleavage of the N-O bond results in a turnon response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(II) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.