文献类型：期刊文献

英文题名：Enhanced liquid–liquid phase separation of stress granules in a reconstructed model and their cytoplasmic targeting using a DNA nanodevice

作者：Liao, Yue[1]; Fan, Chunyu[1]; Zheng, Jiaxin[1]; Liu, Caixia[1]; Zhu, Weiping[1,3]; Xu, Yufang[1]; Qian, Xuhong[1,2]; Yang, Yangyang[1]

机构：[1] Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China; [2] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; [3] Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China

年份：2024

卷号：13

期号：5

起止页码：1744

外文期刊名：Journal of Materials Chemistry B

收录：EI(收录号：20245217589683)

语种：英文

外文关键词：Granulation - Nucleic acid sequences - RNA

摘要：Biomolecular condensates (BCs) are crucial membraneless organelles formed through the process of liquid–liquid phase separation (LLPS) involving proteins and nucleic acids. These LLPS processes are tightly linked with essential cellular activities. Stress granules (SGs), functioning as cytoplasmic BCs, play indispensable roles in maintaining cellular homeostasis and are implicated in diseases like cancers and neurodegenerative disorders. However, devices that can regulate SG LLPS are lacking. Herein, a triangular prism-shaped DNA nanostructure containing polythymidine (DDNA(polyT)) is presented as a nanodevice to investigate the LLPS process of in vitro reconstructed SGs (rSGs), a mixture of marker protein G3BP1 and total RNAs. Our observations reveal that the concentration threshold required for rSG LLPS decreases upon addition of DDNA(polyT), suggesting an enhancement in SG LLPS efficiency. It is speculated that DDNA(polyT) can concentrate mRNAs onto its surface via polyT hybridization with poly-adenosine sequences (polyA) in mRNAs. This alteration in the spatial distribution of mRNAs subsequently affects the multivalency interactions between G3BP1 and mRNAs. Furthermore, DDNA(polyT) exhibits excellent colocalization with cytoplasmic SGs under stressed conditions. This DNA-based nanodevice presents a new artificial approach for the targeted regulation of BC LLPS and holds promise for future studies focusing on BCs. ? The Royal Society of Chemistry 2025.