文献类型：期刊文献

英文题名：Rational Design of Ratiometric Near-Infrared Aza-BODIPY-Based Fluorescent Probe for in Vivo Imaging of Endogenous Hydrogen Peroxide

作者：Mao, Wenle[1];Zhu, Mingming[2,3,4,5];Yan, Chenxu[1];Ma, Yiyu[1];Guo, Zhiqian[1];Zhu, Weihong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, State Key Lab Bioreactor Engn,Shanghai Key Lab Fu, Shanghai 200237, Peoples R China;[2]Minist Hlth, Key Lab Gastroenterol & Hepatol, Div Gastroenterol & Hepatol, 145 Middle Shandong Rd, Shanghai 200001, Peoples R China;[3]Shanghai Inflammatory Bowel Dis Res Ctr, 145 Middle Shandong Rd, Shanghai 200001, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, 145 Middle Shandong Rd, Shanghai 200001, Peoples R China;[5]Shanghai Inst Digest Dis, 145 Middle Shandong Rd, Shanghai 200001, Peoples R China

年份：2020

卷号：3

期号：1

起止页码：45

外文期刊名：ACS APPLIED BIO MATERIALS

收录：EI(收录号：20194707710555);WOS:【ESCI(收录号:WOS:000606759900007)】；

基金：This work was supported by the National Natural Science Foundation of China (21622602, 21788102, 21421004, 21636002, and 21908060), National Key Research and Development Program (2017YFC0906902), Shanghai Municipal Science and Technology Major Project (2018SHZDZX03), Innovation Program of Shanghai Municipal Education Commission, Scientific Committee of Shanghai (15XD1501400), the Shuguang Program (185G27), and China Postdoctoral Science Foundation (2019M651417).

语种：英文

外文关键词：fluorescent probe; near-infrared; ratiometric; aza-BODIPY; in vivo imaging

摘要：Precise in vivo tracking of hydrogen peroxide is still challenging due to its dynamic complexity and intrinsic background interference. Herein, we describe a rational design strategy to construct asymmetric aza-boron-dipyrromethane derivative (BODIPY)-based ratiometric probes for in vivo tracking H2O2, which are composed of a near-infrared aza-BODIPY core, active targeting group, and H2O2-specific recognition unit. We take advantage of two terminal functionalized conjunctions in the bis-condensed aza-BODIPY by rationally introducing carbonyl group as an electrondeficiency linker for regulating intramolecular charge transfer-induced wavelength shift and by attaching hydrophilic polyethylene glycol-biotin segment as the active targeting moiety. The probe BP5-NB-OB features several striking characteristics: (i) ratiometric near infrared response in both absorption and emission spectra; (ii) active targeting ability (biotin receptor-mediated endocytosis) with excellent biocompatibility; and (iii) in vivo tracking of endogenous H2O2. It was demonstrated that the probe BP5-NB-OB was successfully utilized for tracking endogenous H2O2 in living cells and tumorbearing mice, providing opportunities to insight into H2O2 related diseases for clinical application.