文献类型：期刊文献

英文题名：Silver-amplified fluorescence immunoassay via aggregation-induced emission for detection of disease biomarker

作者：Sun, Ze-Hui[1];Zhang, Xuan-Xuan[1];Xu, Duo[1];Liu, Jie[1];Yu, Ru-Jia[1];Jing, Chao[1];Han, Huan-Xing[2];Ma, Wei[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Second Mil Med Univ, Changzheng Hosp, Translat Med Ctr, Shanghai, Peoples R China

年份：2021

卷号：225

外文期刊名：TALANTA

收录：;EI(收录号：20205109650960);WOS:【SCI-EXPANDED(收录号:WOS:000652032600031)】；

基金：This research was supported by Shanghai Pujiang Program (2019PJD010), National Natural Science Foundation of China (21775043), Shanghai Municipal Natural Science Fund (19ZR1472100), Xiamen University Opening Project of PCOSS (201901), Fundamental Research Funds for the Central Universities (JKJ012016013), Shanghai Sailing Program (18YF1405700) and Shanghai Strategic Emerging Industry Major Project (AI/RD2017-03).

语种：英文

外文关键词：Disease biomarker; Fluorescence immunoassay; Enzyme-free amplification; Aggregation induced emission; Biosensor

摘要：Development of simple, robust, and reliable detection strategy of disease biomarkers holds tremendous promise for early clinical diagnosis and prognosis of diseases. In this work, through combining a silver nanoparticle (AgNP) linked immunoassay and aggregation induced emission (AIE)-based fluorogenic Ag+ probe, we developed a silver-amplified fluorescence immunoassay for the detection of disease biomarkers. This method overcame the intrinsic limitations of enzymes as the dissolution of AgNPs generated numerous Ag+, which could switch on the fluorogenic Ag+ probe driven by tetrazolate-Ag+ complexation. As a proof of concept, our method could be used for determining alpha-fetoprotein (AFP) with a linear relationship in concentrations ranging from 0.1 ng mL(-1) to 5 mu g mL(-1) and a low limit of detection of 42 pg mL(-1). Our method was successfully confirmed for the detection of AFP in real serum samples from hepatocellular carcinoma (HCC) patients, demonstrating the great potential for clinical diagnosis.