文献类型：期刊文献

中文题名：聚电解质微球多级自组装制备双重药物载体

英文题名：DUAL DRUG DELIVERY SYSTEM FROM MULTI-STEP SELF-ASSEMBLED POLYELECTROLYTE NANOPARTICLES

作者：卫兰[1];蔡春华[1];林嘉平[1]

机构：[1]华东理工大学材料科学与工程学院上海市先进聚合物材料重点实验室,上海200237

年份：2011

卷号：21

期号：12

起止页码：1461

中文期刊名：高分子学报

外文期刊名：Acta Polymerica Sinica

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000299018300015)】；北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(基金号50925308)资助项目

语种：中文

中文关键词：壳聚糖;胶束;静电自组装;多级自组装;双重载药

外文关键词：Chitosan; Micelles; Electrostatic self-assembly; Multi-step self-assembly; Dual-drug delivery

摘要：将表面带正电荷的壳聚糖(CS)微球和表面带负电荷的聚(L-谷氨酸)-b-聚氧化丙烯-b-聚(L-谷氨酸)(GPG)胶束共混,制备了CS/GPG聚集体水溶液体系.通过改变CS/GPG的共混比例,研究了CS微球和GPG胶束形成稳定CS/GPG聚集体水溶液体系的配比范围,并对其粒径分布和表面电位进行了表征.在此基础上,将CS微球作为亲水性药物阿司匹林(Asp)的载体,GPG胶束作为疏水性药物阿霉素(DOX)的载体,通过多级自组装形成一种新型双重载药体系.研究了该双重药物载体的体外药物释放行为.结果表明,Asp和DOX都具有很好的药物缓释效果,并且2种药物的释药行为都具有明显的pH值响应性.
Self-assembly of chitosan（CS） particles and poly（L-glutamic acid）-b-poly（propylene oxide）-b-poly（L-glutamic acid）（GPG） triblock copolymer micelles was investigated.The CS particles are cationic hydrogels,while the GPG micelles have anionic poly（L-glutamic acid） shells.Therefore,the two kinds of polyelectrolyte particles can electrostatic self-assemble into microscopic CS/GPG conjugates.The CS/GPG ratio range for the CS particles and GPG micelles to electrostatic self-assemble into a sustained CS/GPG conjugates solution was established.The CS/GPG conjugates were prepared by dropping CS particle solution into GPG micelle solution under stirring,with the CS/GPG ratio of 0.25/10,0.5/10,0.75/10,1.0/10,1.25/10,1.5/10,1.75/10 and 2/10.The CS/GPG conjugates are stable in water when the CS/GPG ratio is equal or less than 1.5/10.The particle size distribution of GPG micelles,CS particles,and CS/GPG conjugates were determined by the dynamic light scattering.GPG micelles show a narrow distribution with the average Rh of 59 nm,while the CS particles and CS/GPG conjugates show much broaden distributions with the Rh of 177 nm and 364 nm respectively.Zeta potential tests show that the GPG micelles exhibit surface negative charge of-52 mV and the CS particles exhibit surface positive charge of 22 mV.The surface charge of CS/GPG conjugates are negative and the average absolute value of the surface charge decreases with increasing CS amount.In vitro drug release behaviors of the Aspirin（Asp）-loaded CS particles,Doxorubicin（DOX）-loaded GPG micelles,and the Asp-CS/DOX-GPG conjugates（CS/GPG=1.5/10） were studied as a function of pH value.Both Asp and DOX show pH sensitive release behaviors in all the three drug-loaded systems.Moreover,the release rate of Asp shows a dramatic decrease in the Asp-CS/DOX-GPG system,as compared with the Asp-CS nanoparticles.