文献类型：期刊文献

英文题名：Profiling metabolic states with genetically encoded fluorescent biosensors for NADH

作者：Zhao, Yuzheng[1,2];Yang, Yi[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2015

卷号：31

起止页码：86

外文期刊名：CURRENT OPINION IN BIOTECHNOLOGY

收录：;EI(收录号：20150400445030);WOS:【SCI-EXPANDED(收录号:WOS:000348960200013)】；

基金：Supported by the 973 Program (2013CB531200), NSFC (31225008, 91313301, 31071260, and 31170815), Specialized Research Fund for the Doctoral Program of Higher Education (20100074110010), Shanghai Science and Technology Commission (12JC1402900, 14XD1401400, and 11DZ2260600), Dawn Program of Shanghai Education Commission (11SG31), the 111 Project (B07023), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Metabolism

摘要：NADH and its oxidized form, NAD(+), play central roles in energy metabolism and are ideal indicators of cellular metabolic states. In this review, we will introduce recent progress made in the developing of a series of genetically encoded NADH sensors, which offer the potential to fill the gap in currently used techniques of endogenous NAD(P)H fluorescence imaging. These sensors are bright, specific and organelles targetable, allowing real-time tracking and quantification of intracellular NADH levels in different subcellular compartments. The individual strengths and weaknesses of these sensors when applied to the study of metabolic states profiling will be also discussed.