文献类型：期刊文献

英文题名：Photocrosslinked natural hydrogel composed of hyaluronic acid and gelatin enhances cartilage regeneration of decellularized trachea matrix

作者：Xu, Yong[2];Wang, Zongxin[1,3,4];Hua, Yujie[3,4,5];Zhu, Xinsheng[2];Wang, Yahui[1];Duan, Liang[2];Zhu, Linyong[5];Jiang, Gening[2];Xia, Huitang[1,3,4];She, Yunlang[2];Zhou, Guangdong[1,3,4]

机构：[1]Weifang Med Univ, Res Inst Plast Surg, Weifang, Shandong, Peoples R China;[2]Tongji Univ, Shanghai Pulm Hosp, Dept Thorac Surg, Sch Med, Shanghai, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Med, Shanghai Key Lab Tissue Engn, Dept Plast & Reconstruct Surg,Shanghai Peoples Ho, Shanghai, Peoples R China;[4]Natl Tissue Engn Ctr China, Shanghai, Peoples R China;[5]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China

年份：2021

卷号：120

外文期刊名：MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20204609488376);WOS:【SCI-EXPANDED(收录号:WOS:000617996600004)】；

基金：This work was supported by the National Key Research and Development Program of China (2017YFC1103900), the National Natural Science Foundation of China (81571823, 81771568, 31700837, 81270070, 81701843, 81970014, and 82070022), the Key Research and Development Program of Shandong Province (2016GGB14002), the Shanghai Committee of Science and Technology (15DZ1941600), the Program of Shanghai Technology Research Leader, and the Program for Shanghai Outstanding Medical Academic Leader.

语种：英文

外文关键词：Photocrosslinked natural hydrogel; Decellularized trachea matrix; Laser micropore technique; Cartilage regeneration; Tissue engineered trachea

摘要：Repair of long segmental trachea defects is always a great challenge in the clinic. The key to solving this problem is to develop an ideal trachea substitute with biological function. Using of a decellularized trachea matrix based on laser micropore technique (LDTM) demonstrated the possibility of preparing ideal trachea substitutes with tubular shape and satisfactory cartilage regeneration for tissue-engineered trachea regeneration. However, as a result of the very low cell adhesion of LDTM, an overly high concentration of seeding cell is required, which greatly restricts its clinical translation. To address this issue, the current study proposed a novel strategy using a photocrosslinked natural hydrogel (PNH) carrier to enhance cell retention efficiency and improve tracheal cartilage regeneration. Our results demonstrated that PNH underwent a rapid liquid-solid phase conversion under ultraviolet light. Moreover, the photo-generated aldehyde groups in PNH could rapidly react with inherent amino groups on LDTM surfaces to form imine bonds, which efficiently immobilized the cell-PNH composite to the surfaces of LDTM and/or maintained the composite in the LDTM micropores. Therefore, PNH significantly enhanced cell-seeding efficiency and achieved both stable cell retention and homogenous cell distribution throughout the LDTM. Moreover, PNH exhibited excellent biocompatibility and low cytotoxicity, and provided a natural three-dimensional biomimetic microenvironment to efficiently promote chondrocyte survival and proliferation, extracellular matrix production, and cartilage regeneration. Most importantly, at a relatively low cell-seeding concentration, homogeneous tubular cartilage was successfully regenerated with an accurate tracheal shape, sufficient mechanical strength, good elasticity, typical lacuna structure, and cartilage-specific extracellular matrix deposition. Our findings establish a versatile and efficient cell-seeding strategy for regeneration of various tissue and provide a satisfactory trachea substitute for repair and functional reconstruction of long segmental tracheal defects.