文献类型：期刊文献

英文题名：Efficient ilamycins production utilizing Enteromorpha prolifera by metabolically engineered Streptomyces atratus

作者：Jiang, Yu-Xi[1];Zheng, Gao-Fan[1];Chen, Long-Chao[1];Yang, Na[1];Xin, Xiu-Juan[1];Ma, Jun-Ying[3];Ju, Jian-Hua[3];Wu, Hui[1];Zhao, Ming[5];Wang, Ruida[1,2];An, Fa-Liang[1,4]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, Dept Appl Biol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol,Guangdong Key Lab Marin, Guangzhou 528225, Peoples R China;[4]Marine Biomed Sci & Technol Innovat Platform Lin, 4, Lane 218,Haiji Sixth Rd, Shanghai 201306, Peoples R China;[5]Anhui Polytech Univ, Coll Biol & Food Engn, Anhui Engn Lab Ind Microbiol Mol Breeding, Wuhu 241000, Peoples R China

年份：2023

卷号：16

期号：1

外文期刊名：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

收录：;EI(收录号：20234114876449);WOS:【SCI-EXPANDED(收录号:WOS:001078865700001)】；

基金：We thank Professor Haizhen Wu from East China University of Science and Technology for kindly providing us with the strain, E. coli DH5 alpha and E. coli S17-1. We also thank Professor Xudong Qu, from Shanghai Jiao Tong university for kindly providing us with the plasmid pWHU2449.

语种：英文

外文关键词：Ilamycins; Anti-tuberculosis; Streptomyces atratus SCSIO ZH16; Entromorpha prolifera; BldD

摘要：With the invasion of green tides and the increase of urban green areas worldwide, multimillion tons of Enteromorpha need to be reutilized. In this study, Enteromorpha prolifera powder is considered a promising biomass resource for the production of commercial chemical products production. Ilamycins, novel cyclic heptapeptides with significant anti-TB activities, are isolated from Streptomyces atratus SCSIO ZH16, a deep-sea-derived strain. Using EP powder as a nitrogen source, the production of ilamycins reached 709.97 mg/L through optimization of the nitrogen source using the engineered strain S. atratus SCSIO ZH16 Delta R. After mutant strain constructions and tests, strain S. atratus SCSIO ZH16 Delta R::bldD EP powder achieved a higher production titer of ilamycins. Furthermore, the production titer of ilamycins and ilamycin E reached 1561.77 mg/L and 745.44 mg/L, respectively, in a 5 L bioreactor. This study suggests that E. prolifera is a promising and eco-friendly nitrogen source for the production of ilamycins.