文献类型：期刊文献

英文题名：A Fluorescence-SERS Dual-Mode Nanoprobe for Imaging of HSP90 mRNA and Peroxynitrite in Living Cells

作者：He, Yue[1];Ye, Ming-Jie[1];Xi, Cheng-Ye[1];Yu, Jun-Jie[1];Chen, Bin-Bin[1];Chen, Hua-Ying[2];Li, Da-Wei[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Zhejiang Shuren Univ, Coll Biol & Environm Engn, Hangzhou 310015, Peoples R China

年份：2025

卷号：10

期号：5

起止页码：3737

外文期刊名：ACS SENSORS

收录：;EI(收录号：20251918392220);WOS:【SCI-EXPANDED(收录号:WOS:001485380900001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Grant No. 22176058), Science and Technology Commission of Shanghai Municipality (24DX1400200), the Program of Introducing Talents of Discipline to Universities (B16017), the Fundamental Research Funds for the Central Universities (222201717003), and Talent Introduction Project of Zhejiang Shuren University (2023R070). The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help with the characterization.

语种：英文

外文关键词：SERS; fluorescence; nanoprobe; HSP90mRNA; ONOO-; cell imaging

摘要：The dysregulation of heat shock protein 90 mRNA (HSP90 mRNA) and reactive oxygen species (ROS) is implicated in stress response and various diseases. Visualizing HSP90 mRNA and ROS dynamics is important to studying their interactions and related physiopathological mechanisms. However, effective methods for detecting both remain lacking. Herein, a covalent organic framework-based (COF-based) dual-mode nanoprobe is designed to monitor HSP90 mRNA and ONOO- (ROS model). The nanoprobe is prepared by in situ assembly of a COF shell as the aptamer carrier on the gold nanorods (AuNRs), followed by conjugation of the ONOO--responsive molecule, 4-mercaptophenylboronic acid (4-MPBA), to the AuNRs and modification of the HSP90 mRNA aptamer (HSP90MB) onto the COF shell. The prepared nanoprobe enables sensitive and selective fluorescence (FL) and surface-enhanced Raman spectroscopy (SERS) detection of HSP90 mRNA and ONOO-, respectively. The dual-channel detection highlights the advantages of facilitating spectral analysis and eliminating mutual interference. In addition, the proposed strategy visualizes a positive interaction between HSP90 mRNA and ONOO- in living cells, revealing their cellular response mechanism under stress conditions and related diseases.