文献类型：期刊文献

英文题名：Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells

作者：Jiang, Li[1,2,3];Xie, Xin[1,2];Su, Ni[1,2];Zhang, Dasheng[1,4];Chen, Xianjun[1,2];Xu, Xiaochen[5];Zhang, Bibi[1,2];Huang, Kaiyi[5,6];Yu, Jingwei[1,2];Fang, Mengyue[1,2];Bao, Bingkun[1,2,3];Zuo, Fangting[1,2];Yang, Lipeng[1,2,3];Zhang, Rui[1,2];Li, Huiwen[1,2];Huang, Xinyi[1,2,3];Chen, Zhengda[1,2];Zeng, Qingmei[1,2,3];Liu, Renmei[1,2];Lin, Qiuning[3];Zhao, Yuzheng[1,2];Ren, Aiming[5,6];Zhu, Linyong[1,2,3,5];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ct, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Me, Sch Pharm, Shanghai, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai, Peoples R China;[4]Fluorescence Diag Shanghai Biotech Co Ltd, Shanghai, Peoples R China;[5]Zhejiang Univ, Inst Life Sci, Hangzhou, Peoples R China;[6]Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Orthoped Surg, Hangzhou, Peoples R China

年份：2023

卷号：20

期号：10

起止页码：1563

外文期刊名：NATURE METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001096071500021)】；

基金：This research was supported by the National Key Research and Development Program of China (2022YFC3400100 to Y.Y. and X.C., 2019YFA0110500 to L.Z., 2019YFA0904800 to Y.Y. and L.Z. and 2021YFC2300300 to A.R.), NSFC (91857202, 32121005, 32150028 and 21937004 to Y.Y.; 21907029 and 21877037 to L.Z.; 32022039, 31870810, 91940302 and 91640104 to A.R.; 32250009 to X.C.; 32001051 to N.S.), STI2030-Major Projects (2021ZD0202200 and 2021ZD0202203 to X.C.), the Shanghai Municipal Education Commission (2021 Sci and Tech 03-28), ALS Project from Shanghai Jiao Tong University School of Medicine Affiliated Sixth People's Hospital (to Y.Y.), the Shanghai Rising-Star Program to X.C., the State Key Laboratory of Bioreactor Engineering (to Y.Y.), China Postdoctoral Science Foundation (2022M711147 to X. Xie.), and the Fundamental Research Funds for the Central Universities (to Y.Y. and X.C.).

语种：英文

摘要：Fluorescent RNAs, aptamers that bind and activate small fluorogenic dyes, have provided a particularly attractive approach to visualizing RNAs in live cells. However, the simultaneous imaging of multiple RNAs remains challenging due to a lack of bright and stable fluorescent RNAs with bio-orthogonality and suitable spectral properties. Here, we develop the Clivias, a series of small, monomeric and stable orange-to-red fluorescent RNAs with large Stokes shifts of up to 108 nm, enabling the simple and robust imaging of RNA with minimal perturbation of the target RNA's localization and functionality. In combination with Pepper fluorescent RNAs, the Clivias enable the single-excitation two-emission dual-color imaging of cellular RNAs and genomic loci. Clivias can also be used to detect RNA-protein interactions by bioluminescent imaging both in live cells and in vivo. We believe that these large Stokes shift fluorescent RNAs will be useful tools for the tracking and quantification of multiple RNAs in diverse biological processes.