文献类型：期刊文献

英文题名：Preparation of immunomagnetic nanoparticles and their application in the separation of mouse CD34+ hematopoietic stem cells

作者：Liang, Xin[1]; Xu, Ke[1]; Xu, Jianhe[1]; Chen, Wei[2]; Shen, Hebai[2]; Liu, Jianwen[1]

机构：[1] School of Pharmacy, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] Life and Environment Science College, Shanghai Normal University, Shanghai, 200234, China

年份：2009

卷号：321

期号：12

起止页码：1885

外文期刊名：Journal of Magnetism and Magnetic Materials

收录：EI(收录号：20091612043455)

语种：英文

外文关键词：Magnetic materials - Stem cells - Nanomagnetics - Cytology - Monoclonal antibodies - Transmission electron microscopy - Separation - Synthesis (chemical) - Mammals - Nanoparticles - Scanning electron microscopy

摘要：The magnetic nanoparticles with a diameter of about 60 nm were synthesized by coprecipitation from ferrous and ferric iron solutions and coated with silica. Then the nanoparticles were modified with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPS) in order to immobilize anti-CD34+ monoclonal antibodies to the surface of modified magnetic particles. The results of transmission electron microscope (TEM) and Fourier transformed infrared (FT-IR) indicated that the nanoparticles were successfully prepared. Scanning electron microscope (SEM) photo confirmed that the mouse CD34+ cells (cells expressing CD34) were separated by the immunomagnetic nanoparticles. The viability of the separated cells was studied by hematopoietic colony-forming assay, the result of which showed that the target cells still had an ability of proliferation and differentiation. The application of the separated CD34+ cells was in testing the pharmacological effect of three samples isolated from enzyme-digested traditional Chinese medicine Colla corii asini. Crown Copyright ? 2008.