文献类型：期刊文献

英文题名：Label-free electrochemical impedance spectroscopy biosensor for direct detection of cancer cells based on the interaction between carbohydrate and lectin

作者：Hu, Yaofang[1];Zuo, Peng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2013

卷号：43

期号：1

起止页码：79

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20130215880832);WOS:【SCI-EXPANDED(收录号:WOS:000316525300014)】；

基金：This work was financially supported by National Natural Science Foundation of China (21075040, 31101285), the Shanghai Fund (11nm0502500, 11XD1401900), the Key Grant Project of Chinese Ministry of Education (No. 313019), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Electrochemical impedance spectroscopy; Label free; Cancer cell detection; Carbohydrate and lectin interaction; Lectin sensor

摘要：A novel label-free electrochemical impedance spectroscopy (EIS) biosensor for direct cancer cell detection based on the interaction between carbohydrate and lectin has been developed with good sensitivity and selectivity. In the present work, concanavalin A (Con A), a mannose specific lectin, was immobilized on a gold disk electrode to fabricate the Con A sensor. This sensor was incubated with the cancer cell sample, and the binding of cancer cells with Con A resulted in a change of charge transfer resistance (R-ct). EIS measurement was employed to measure the impedance change which reveals the concentration of cancer cells. This method has been successfully applied in human liver cancer cell Bel-7404 for direct and sensitive detection with a detection limit of 234 cells/mL. This method could be extended to carry out multi-component diagnosis applications, thus providing enormous potential for applications of cancer monitoring and therapy. (c) 2012 Elsevier B.V. All rights reserved.