详细信息
文献类型:期刊文献
中文题名:阿维菌素在土壤中的微生物降解研究
英文题名:Microbial degradation of Abamectin in soil.
作者:张卫[1];虞云龙[2];林匡飞[1];李少南[2];吴加伦[2];樊德方[2]
机构:[1]华东理工大学资环学院,上海200237;[2]浙江大学农药环境毒理研究所,杭州310029
年份:2004
卷号:15
期号:11
起止页码:2175
中文期刊名:应用生态学报
外文期刊名:Chinese Journal of Applied Ecology
收录:MEDLINE(收录号:15707337);CSTPCD;;Scopus;CSCD:【CSCD2011_2012】;PubMed;
基金:国家自然科学基金资助项目 (3 0 2 70 880 )
语种:中文
中文关键词:阿维菌素;土壤;微生物降解;优势菌;粗酶液
外文关键词:Abamectin, Soil, Microbial degradation, Dominant bacteria, Crude enzyme.
摘要:运用恒温培养法研究了阿维菌素在土壤中的降解动力学 .结果表明 ,非生物 +微生物降解、非生物降解及微生物降解的半衰期分别为 34 8、2 77 3和 4 9 9d ,说明阿维菌素在土壤中的降解主要由微生物引起 .从试验土壤中分离到 1株高效降解阿维菌素的菌株 ,经 16SrDNA鉴定为嗜麦芽寡养单胞菌(Stenotrophomonasmaltrophilia) .从该降解菌中提取的粗酶液米氏常数 (Km)为 6 78nmol·ml-1,最大降解速率为 81 5nmol·min-1·mg-1.
Incubation test on the degradation dynamics of Abamectin in soil showed that the half-life of its non-biodegradation plus microbial biodegradation,non-biodegradation,and microbial biodegradation was 34.8,277.3 and 49.9 d,respectively,and its degradation in soil was mostly by microbes.A dominant bacterium which could effectively degrade Abamectin was isolated from test soil,and identified as Stenotrophomonas maltrophilia by 16S rDNA.The crude enzyme extracted from the dominant bacteria had a Michaelis-Mentn's constant 6.78 nmol·ml -1 and a maximum rate 81.5 nmol·min -1 ·mg -1 .
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