文献类型：期刊文献

英文题名：Nitrogen regulator GInR controls uptake and utilization of non-phosphotransferase-system carbon sources in actinomycetes

作者：Liao, Cheng-Heng[1];Yao, Lili[1];Xu, Ya[1];Liu, Wei-Bing[1];Zhou, Ying[1];Ye, Bang-Ce[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, Xinjiang 832000, Peoples R China

年份：2015

卷号：112

期号：51

起止页码：15630

外文期刊名：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000366916000043)】；

基金：We thank Sebastien Rigali (University of Liege) for his kind assistance with this work and Wolfgang Wohlleben (University of Tuebingen) for providing the S. coelicolor Delta glnR mutant strain. This work was supported by China Natural National Science Foundation Grants 21276079, 21421004, and 21335003; Chinese Ministry of Education Grant SRFDP 20120074110009; National Key Technologies R&D Programs 2014AA021502; and by Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：actinomycetes; GInR; carbon sources utilization; ABC transport system; nitrogen metabolism

摘要：The regulatory mechanisms underlying the uptake and utilization of multiple types of carbohydrates in actinomycetes remain poorly understood. In this study, we show that GlnR (central regulator of nitrogen metabolism) serves as a universal regulator of nitrogen metabolism and plays an important, previously unknown role in controlling the transport of non-phosphotransferase-system (PTS) carbon sources in actinomycetes. It was observed that GlnR can directly interact with the promoters of most (13 of 20) carbohydrate ATP-binding cassette (ABC) transporter loci and can activate the transcription of these genes in response to nitrogen availability in industrial, erythromycin-producing Saccharopolyspora erythraea. Deletion of the glnR gene resulted in severe growth retardation under the culture conditions used, with select ABC-transported carbohydrates (maltose, sorbitol, mannitol, cellobiose, trehalose, or mannose) used as the sole carbon source. Furthermore, we found that GlnR-mediated regulation of carbohydrate transport was highly conserved in actinomycetes. These results demonstrate that GlnR serves a role beyond nitrogen metabolism, mediating critical functions in carbon metabolism and crosstalk of nitrogen-and carbon-metabolism pathways in response to the nutritional states of cells. These findings provide insights into the molecular regulation of transport and metabolism of non-PTS carbohydrates and reveal potential applications for the cofermentation of biomass-derived sugars in the production of biofuels and bio-based chemicals.