文献类型：期刊文献

英文题名：Hierarchically microporous/macroporous scaffold of magnesium-calcium phosphate for bone tissue regeneration

作者：Wei, Jie[3,4];Jia, Junfeng[3,4];Wu, Fan[3,4];Wei, Shicheng[1,2,6];Zhou, Huanjun[3,4];Zhang, Hongbo[3,4];Shin, Jung-Woog[5];Liu, Changsheng[3,4]

机构：[1]Peking Univ, Dept Oral & Maxillofacial Surg, Sch Stomatol, Beijing 100081, Peoples R China;[2]Peking Univ, Hosp Stomatol, Beijing 100081, Peoples R China;[3]E China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[5]Inje Univ, Dept Biomed Engn, Res Grp 1, BK 21, Gimhae 621749, South Korea;[6]Peking Univ, Ctr Biomed Mat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

年份：2010

卷号：31

期号：6

起止页码：1260

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20095212572645);WOS:【SCI-EXPANDED(收录号:WOS:000273985100027)】；

基金：The authors appreciate financial support from the National Natural Science Funds of China (No. 30970720), Nano special program of Science and Technology Development of Shanghai (No. 0852nm02700), Standard Special program of Science and Technology Development of Shanghai (No. 08DZ0501600), the 973Project of the Ministry of Science and Technology of the People's Republic of China (Grant 2007CB936103) and Sponsored by Shanghai Pujiang Program (2008).

语种：英文

外文关键词：Hierarchically porous scaffolds; Magnesium calcium phosphate; Biodegradability; Biocompatibility; Bone generation

摘要：Hierarchically 3D microporous/macroporous magnesium-calcium phosphate (micro/ma-MCP) scaffolds containing magnesium ammonium phosphate hexahydrate [NH4MgPO4 center dot 6H(2)O] and hydroxyapatite [Ca-10(PO4)(6)(CH)(2)] were fabricated from cement utilizing leaching method in the presence of sodium chloride (NaCl) particles and NaCl saturated water solution. NaCl particles produced macropororosity, and NaCl solution acted as both cement liquid and porogens, inducing the formation of microporosity. The micro/ma-MCP scaffolds with porosities varied from 52 to 78% showed well interconnected and open macropores with the sizes of 400-500 mu m, and degradation of the scaffolds was significantly enhanced in Tris-HCl solution compared with macroporous MCP (ma-MCP) and corresponding calcium phosphate cement (CPC) scaffolds. Cell attachment and proliferation of MG(63) on micro/ma-MCP were significantly better than ma-MCP and CPC scaffolds because of the presence of microporosity, which enhanced the surface area of the scaffolds. Moreover, the alkaline phosphatase (ALP) activity of the MG(63) cells on micro/ma-MCP was significantly higher than ma-MCP and CPC scaffolds at 7 days, and the MG(63) cells with normal phenotype spread well and formed confluent layers across the macroporous walls of the microlma-MCP scaffolds. Histological evaluation confirmed that the micro/ma-MCP scaffolds improved the efficiency of new bone regeneration, and exhibited excellent biocompatibility, biodegradability and faster and more effective osteogenesis in vivo. (C) 2009 Elsevier Ltd. All rights reserved.