文献类型：期刊文献

中文题名：尿激酶亲和层析分离中吸附和洗脱过程的速率模型模拟

英文题名：Rate Model Simulation of Adsorption and Step Elution of Urokinase Using Affinity Chromatography

作者：朱家文[1];武斌[1];陈葵[1]

机构：[1]华东理工大学化学工程研究所,上海200237

年份：2003

卷号：29

期号：2

起止页码：109

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2003257514396);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目(29976014)

语种：中文

中文关键词：速率模型;亲和层析;穿透曲线;尿激酶;吸附等温线;吸附动力学;洗脱过程;模拟计算

外文关键词：rate model simulation; affinity chromatography; breakthrough; elution; urokinase

摘要：采用非平衡层析速率模型,对尿激酶的亲和层析过程进行了模拟计算。利用亲和层析,可以将粗品原料净化为高纯度尿激酶产品;层析所用的亲和载体是采用对氨基苯甲脒作为配基,将琼脂糖凝胶进行活化制得的。通过间歇实验,测定了吸附等温线并进行吸附动力学研究。借助于VERSE-LC模拟软件,采用速率模型作为基本方程,对亲和层析的实验现象进行了数值模拟分析,与实验结果进行对比发现,速率模型可以较准确地描述固定床中的亲和层析过程。
A nonequilibrium chromatographic rate model was employed to simulate the affinity chromatographic process of urokinase. The chromatographic process was developed to yield a high purity product of urokinase from crude materials. The affinity gel used in the process was prepared by an epichlorohydrinactivation method using epichlorohydrin activated Sepharose 4B as a matrix and paminobenzamidine as a ligand. Adsorption kinetics and isotherm were measured in batchwise operation. The experimental phenomena in the chromatography were numerically simulated and analyzed with the aid of VERSELC computer simulator. Rate model was utilized as basic principles in simulation. Comparison of the simulation results with the experimental data shows that the proposed rate model can be used to describe the affinity chromatography of urokinase in a fixed bed column with satisfactory accuracy.