文献类型：期刊文献

英文题名：Coordinated response of photosynthesis, carbon assimilation, and triacylglycerol accumulation to nitrogen starvation in the marine microalgae Isochrysis zhangjiangensis (Haptophyta)

作者：Wang, Hai-Tao[1,2];Meng, Ying-Ying[1,3];Cao, Xu-Peng[1];Ai, Jiang-Ning[1];Zhou, Jian-Nan[1];Xue, Song[1];Wang, Wei-liang[4]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Marine Bioengn Grp, Dalian 116023, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China;[4]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：177

起止页码：282

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20145100336173);WOS:【SCI-EXPANDED(收录号:WOS:000346647100037)】；

基金：This work was supported by National Key Basic Research Program of China "973 Program" (2011CBA00803), National High Technology Research and Development Program "863" (2012AA052101 and 2014AA022004), Liaoning Provincial Natural Science Foundation (2012010263), the Hundred Talent Program of the Chinese Academy of Sciences (No. A1097) and Knowledge Innovation Programs of Dalian Institute of Chemical Physics, CAS (K2010A3).

语种：英文

外文关键词：Isochrysis zhangjiangensis; Photosynthesis; Triacylglycerols production; Nitrogen limitation

摘要：The photosynthetic performance, carbon assimilation, and triacylglycerol accumulation of Isochrysis zhangjiangensis under nitrogen-deplete conditions were studied to understand the intrinsic correlations between them. The nitrogen-deplete period was divided into two stages based on the photosynthetic parameters. During the first stage, carbon assimilation was not reduced compared with that under favorable conditions. The marked increase in triacylglycerols and the variation in the fatty acid profile suggested that triacylglycerols were mainly derived from de novo synthesized acyl groups. In the second stage, the triacylglycerol content continued increasing while the carbohydrate content decreased from 44.0% to 26.3%. These results indicated that the intracellular conversion of carbohydrates to triacylglycerols occurred. Thus, we propose that sustainable carbon assimilation and incremental triacylglycerol production can be achieved by supplying appropriate amounts of nitrogen in medium to protect the photosynthetic process from severe damage using the photosynthetic parameters as indicators. (C) 2014 Elsevier Ltd. All rights reserved.