文献类型：期刊文献

英文题名：Enhanced thermotolerance of Arabidopsis by chitooligosaccharides-induced CERK1n-ERc fusion gene

作者：Chen, Linxiao[1];Xia, Wei[1];Song, Jinxing[1];Wu, Mengqi[1];Xu, Zhizhen[1];Hu, Xiangyang[2];Zhang, Wenqing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China;[2]Shanghai Univ, Sch Life Sci, Shanghai, Peoples R China

年份：2020

卷号：15

期号：12

外文期刊名：PLANT SIGNALING & BEHAVIOR

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000567618700001)】；

语种：英文

外文关键词：Chitooligosaccharides; CERK1n-ERc; chimeric receptor; heat stress; jasmonic acid

摘要：Heat stress is a major growth-limiting factor for most crops over the world. Chitin elicitor receptor kinase 1 (CERK1) is a chitin/chitooligosaccharides receptor, andERECTA(ER) plays a crucial role in plant resistance to heat stress. In the present study, a chitooligosaccharides-inducedCERK1n-ERcfusion gene was designed and synthesized, in which the extracellular domain and transmembrane domain ofCERK1gene is connected with the response region ofERgene. We successfully constructed theCERK1n-ERcfusion gene by Overlap PCR and introduced it intoArabidopsisbyAgrobacterium-medicatedinfection. Genetically modified (GM) plants had a greater germination rate and germination index, as well as a shorter mean germination time, indicating that they had a better thermotolerance compared with the wild-type (WT) lines under heat stress. Moreover, the GM lines showed a lower level of hydrogen peroxide (H2O2) and relative electrolyte leakage (REL), suggesting that they were in better state compared with the WT plants when exposed to high temperature. UPLC-MS/MS was employed to assess the phytohormone level, suggesting that the GM lines acquired a better thermotolerance via jasmonic acid (JA) signaling pathways. In general, we constructed a COS-induced fusion gene to enhance the thermotolerance ofArabidopsisduring seed germination and postgermination growth.