文献类型：期刊文献

中文题名：钛金属表面微纳结构协同抗菌肽的抗菌性能研究

英文题名：Study on antibacterial properties of titanium metallic surface due to synergistic action of micro/nano-structure and antimicrobial peptides

作者：何宏燕[1,2];郜彭阳[1];乔忠乾[1];屈雪[1];刘昌胜[1,2]

机构：[1]华东理工大学教育部医用生物材料工程研究中心,上海200237;[2]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2018

卷号：32

期号：9

起止页码：1116

中文期刊名：中国修复重建外科杂志

外文期刊名：Chinese Journal of Reparative and Reconstructive Surgery

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；PubMed;

基金：中国科学院-威高研究发展计划(攻关项目[2017]005号);国家自然科学基金面上项目(31570971);国家自然科学基金创新群体项目(51621002);上海市国际科技合作项目(18520710100);国家外国专家局"111"引智项目(B14018)~~

语种：中文

中文关键词：钛;微纳结构;抗菌肽;抗菌性能;植入材料

外文关键词：Titanium;micro/nano-structure;antibacterial peptides;antibacterial property;implant material

摘要：目的探讨通过构建材料表面微纳结构联合涂覆抗菌肽提升钛金属抗菌性能的可行性。方法取钛片进行喷砂酸蚀(sandblasted large-grit acid-etched,SLA)及碱热处理(alkali-heat treatment,AHT),构建微纳结构;然后表面旋涂10、30、50、70、90μg抗菌肽聚合物。扫描电镜观察处理前后钛片表面形貌,能谱仪分析表面C、N、O、Ti 4种元素含量比例。将表面载有不同量抗菌肽聚合物的钛片与金黄色葡萄球菌液共培养,24 h后观察抑菌圈形成情况,并测量抑菌圈直径。另将处理前、SLA处理、SLA+AHT处理、载有90μg抗菌肽聚合物的钛片分别与金黄色葡萄球菌以及大肠杆菌共培养,3 h后扫描电镜观察两种细菌在材料表面的黏附情况,3、6、9、12、24 h测量菌液吸光度(A)值,评价钛片抗菌效果。结果扫描电镜下观察,SLA+AHT处理钛片表面构建包含微米及纳米级孔洞的多级结构;旋涂抗菌肽聚合物后表面孔径<200 nm的孔洞几乎已被覆盖。元素分析显示,随着抗菌肽聚合物旋涂量的增加,C元素含量不断增加;但直至含量达70μg时才检测到N元素。培养24 h后,载有不同量抗菌肽聚合物钛片周围均出现明显抑菌圈,其中90μg钛片大于其余钛片,比较差异均有统计学意义(P<0.05)。抗菌实验结果显示,载抗菌肽聚合物的钛片表面细菌黏附量较其余钛片明显减少,菌液A值明显降低,比较差异有统计学意义(P<0.05)。结论通过在材料表面制备微纳结构并涂覆抗菌肽,可赋予钛金属表面优良的抗菌性能。
Objective To investigate the effects of micro/nano-structure and antimicrobial peptides（AMPs） on antibacterial properties of titanium（Ti） metallic surface. Methods Ti disks were treated via sandblasted large-grit acidetched（SLA） and alkali-heat treatment（AHT） to build the micro/nano-structure, on which AMPs were spin-coated with a certain amount（10, 30, 50, 70, and 90 μg）. Scanning electron microscope（SEM） and energy dispersive spectroscopy（EDS）were used to observe the surface structure and characterize the surface elements（i.e. contents of C, N, O, and Ti）. Ti disks loaded with AMPs of difference amounts were co-cultured with Staphylococcus aureus（S. aureus） for 24 hours. After that,the formation and dimension of antibacterial circle were measured. Furthermore, the Ti disks treated with different approaches（untreated, SLA treatment, SLA＋THA treatment, and 90 μg AMPs-loaded samples） were co-cultured with S. aureus and Escherichia coli（E.coli） for 3 hours, bacterial adhesion on the disks were evaluated by using SEM. The antibacterial performances in solution were quantitatively evaluated by immersing the Ti disks in bacterial solutions and measuring the absorbance（A） values. Results It was found that the nanoporous structure could be easily constructed by SLA＋AHT approach. After spin-coating AMPs, the nanopores with the diameter less than 200 nm were almost covered.According to the element analysis, with the increase of AMPs, the C content gradually increased; the N content was not detected until AMPs amount reached 70 μg on the disks. The diameter of antibacterial circle clearly depended on the AMPs amount. The Ti disks loaded with 90 μg AMPs had significantly larger antibacterial circles than the other Ti disks（P〈0.05）. Based on the SEM observation, the Ti disks loaded with 90 μg AMPs has the least bacterial attachment compared with the other Ti disks（P〈0.05）. The A value of bacterial solution immersed with the Ti disks loaded with 90 μg AMPs was much lower than the other Ti disks（P〈0.05）. Conclusion The approach of micro/nano-structure and AMPs can improve the antibacterial properties of Ti metallic surface.