文献类型：期刊文献

中文题名：指纹图谱技术在茵陈提取工艺优化中的应用

英文题名：Application of HPLC fingerprint to optimization of extraction process of Herba Artemisiae scopariae

作者：王春梅[1];胡坪[1];刘清飞[2];罗国安[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]清华大学药物研究所,北京100084

年份：2007

卷号：29

期号：6

起止页码：785

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市科委中药专项(05DZ19709)资助

语种：中文

中文关键词：茵陈;均匀设计;指纹图谱;提取工艺

外文关键词：Artemisiae scopariae; uniform design; HPLC fingerprint; extraction process

摘要：目的:研究茵陈蒿汤中茵陈药材的最佳提取工艺条件。方法:建立了茵陈的HPLC指纹图谱方法,采用均匀设计法,以溶剂浓度、溶剂用量、提取时间、提取次数4个因素,每个因素9个水平进行提取工艺试验,并采用HPLC指纹图谱技术对各工艺条件下得到的提取物进行分析;最后以指纹图谱相似度与茵陈的提取效率为评价指标确定了茵陈的最佳提取工艺条件。结果:25倍量11.14的乙醇,每次提取90min,提取3次。结论:指纹图谱技术对茵陈提取工艺的优化具有指导意义。
AIM： To optimize the extraction process of Herba Artemisiae scopariae in Yinchenhao Decoction. METHODS ： On the basis of HPLC fingerprint of Herba Artemisiae scopariae uniform design was adopted to the extraction process, with four factors, including solvent concentration, solvent dosage, extraction cyde and extraction times in nine levels. Finally, the best extraction conditions were chosen according to the similarity of HPLC fingerprint and the extraction efficiency. RESULTS： The optimal extract procedure was refluxing for 90 mins with 25 times solvent of 11.14% ethanol for three times. CONCLUSION： HPLC fingerprint is useful for the optimization of extraction process of Herb Artemisiae scopariae.