文献类型：期刊文献

中文题名：启动子cbh1的改造与里氏木霉通用质粒载体的构建

英文题名：Improvement of promoter cbh1 and construction of heterologous gene expression multi-purpose vector of Trichoderma reesei

作者：唐侣洋[1];魏东芝[1];杨忠[1];王玮[1]

机构：[1]华东理工大学鲁华所,上海200237

年份：2014

卷号：44

期号：4

起止页码：33

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：CSCD:【CSCD_E2013_2014】；

基金：国家重点基础研究发展计划(973);项目编号2012CB721103;国家高技术发展计划(863);项目编号2012AA101806

语种：中文

中文关键词：里氏木霉;异源表达;反馈抑制位点;cbh1启动子

外文关键词：Trichoderma reesei; heterologous gene expression; repressor binding site; cbhl promoter

摘要：里氏木霉具有优异的表达及分泌异源蛋白的能力。本研究为了提高里氏木霉在工业生产中异源蛋白表达产量,对cbh1启动子中四处葡萄糖反馈抑制位点进行突变,并且成功构建两株定点插入型表达质粒pG与pH。在后续的报告基因红色荧光蛋白DsRed表达过程中,pG比pH表现出更优异的表达能力,并且异源蛋白基因被定点插入了cbh1基因位点而对该基因进行了敲除。pG质粒具有工业应用前景。
The Trichoderma reesei is an important filamentous fungi due to its ability of expressing and secreting exogenous protein. In order to improve the industrial production of heterologous protein in Trichoderma reesei, four repressor binding sites were mutated and then two plasmids pG and pH were constructed, which could be int reesei. The pG showed more effective than pH when the red fluorescent protein （DsRed） could be integrated at the cbhl locus and then the cbhl was deleted. Plasmid pG showed a egrated at the cbhl locus of T. was used as report gene. pG potential prospect in industrial production.