文献类型：期刊文献

英文题名：Surface Modified with a Host Defense Peptide-Mimicking β-Peptide Polymer Kills Bacteria on Contact with High Efficacy

作者：Qian, Yuxin[1,2,3,4];Qi, Fan[1,2,3,4];Chen, Qi[1,2,3,4];Zhang, Qiang[1,2,3,4];Qiao, Zhongqian[1,2,3,4];Zhang, Si[1,2,3,4];Wei, Ting[7];Yu, Qian[7];Yu, Shan[8];Mao, Zhengwei[8];Gao, Changyou[8];Ding, Yiru[10];Cheng, Yanyong[9];Jin, Chenyu[9];Xie, Hexin[1,5,6];Liu, Runhui[1,2,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Mat Sci & Technol, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[6]East China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[7]Soochow Univ, Coll Chem Chem Engn & Mat Sci, State & Local Joint Engn Lab Novel Funct Polymer, Suzhou 215123, Peoples R China;[8]Zhejiang Univ, Dept Polymer Sci & Engn, MOE Key Lab Macromol Synth & Functionalizat, Hangzhou 310027, Zhejiang, Peoples R China;[9]Shanghai Jiao Tong Univ, Dept Anesthesiol, Sch Med, Shanghai Peoples Hosp 9, Shanghai 200011, Peoples R China;[10]China Pharmaceut Univ, Sch Pharm, Nanjing 210009, Jiangsu, Peoples R China

年份：2018

卷号：10

期号：18

起止页码：15395

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20182005198305);WOS:【SCI-EXPANDED(收录号:WOS:000432205800009)】；

基金：This research was supported by the National Key Research and Development Program of China (2016YFC1100401), the National Natural Science Foundation of China (no. 21574038, 21774031), the National Natural Science Foundation of China for Innovative Research Groups (no. 51621002), the "Eastern Scholar Professorship" from Shanghai local government (TP2014034), the 1000 talent young scholar program in China, 111 project (B14018), the national special fund for State Key Laboratory of Bioreactor Engineering (2060204), the program for professor of special appointment at ECUST, and the MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Zhejiang University (2016MSF04).

语种：英文

外文关键词：beta-peptide polymer; graft to; antimicrobial surface; divalent ion; membrane destabilization; biocompatible surface; antimicrobial resistance; MRSA

摘要：Methicillin-resistant Staphylococcus aureus (MRSA) has been one of the major nosocomial pathogens to cause frequent and serious infections that are associated with various biomedical surfaces. This study demonstrated that surface modified with host defense peptide-mimicking beta-peptide polymer, has surprisingly high bactericidal activities against Escherichia coli (E. coli) and MRSA. As surface-tethered beta-peptide polymers cannot move freely to adopt the collaborative interactions with bacterial membrane and are too short to penetrate the cell envelop, we proposed a mode of action by diffusing away the cell membrane-stabilizing divalent ions, Ca2+ and Mg2+. This hypothesis was supported by our study that Ca2+ and Mg2+ supplementation in the assay medium causes up to 80% loss of bacterial killing efficacy and that the addition of divalent ion chelating ethylenediaminetetraacetic acid into the above assay medium leads to significant recovery of the bacterial killing efficacy. In addition to its potent bacterial killing efficacy, the surface-tethered beta-peptide polymer also demonstrated excellent biocompatibility by displaying no hemolysis and supporting mammalian cell adhesion and growth. In conclusion, this study demonstrated the potential of beta-peptide polymer-modified surface in addressing nosocomial infections that are associated with various surfaces in biomedical applications.