文献类型：期刊文献

英文题名：A new member of the surfactin family produced by Bacillus subtilis with low toxicity on erythrocyte

作者：Fei, Dan[1,2];Liu, Fang-Fang[1,2];Gang, Hong-Ze[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Ye, Ru-Qiang[1,2];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr MEOR, Shanghai 200237, Peoples R China

年份：2020

卷号：94

起止页码：164

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20201808595727);WOS:【SCI-EXPANDED(收录号:WOS:000537690100017)】；

基金：This research was supported by the National High Technology Research and Development Program of China (2013AA064403), the Fundamental Research Funds for the Central Universities of China (22221818014), and Research Program of State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Bacillus subtilis; Microbial lipopeptide; Surfactin; Surface activity; Hemolysis

摘要：A new member of the surfactin family of microbial lipopeptides has been isolated from the cell broth of Bacillus subtilis TD 7 by reverse phase high performance liquid chromatography (RP-HPLC), and identified by FT-IR, GC-MS, LC-MS/MS, H-1-NMR and C-13-NMR. The surfactin-C-11 consists of a heptapeptide with the sequence of N - Glu - Leu - Leu - Val - Asp - Leu - Leu - C, and a beta-hydroxy fatty acid chain with 11 carbons, which is a new member with the shortest hydrophobic chain among the surfactin family produced by B. subtilis. The critical micelle concentration (CMC) of the surfactin-C-11 is 5.59 x 10(-5) mol/L in 10 mmol/L phosphate buffer solution (PBS, pH 7.4) at 25 degrees C, and the surface tension at CMC (gamma(CMC)) is 34.42 mN/m. The hemolytic activities of the surfactin-C-11 on 2 % defibrinated sheep blood showed a HC(50 )of 448.51 mu mol/L. Surfactin with short fatty acid chain has low toxicity on erythrocyte, which implies potential applications in biotechnological and pharmaceutical fields.