文献类型：期刊文献

中文题名：聚(St-DVB)型整体柱的制备及其对蛋白质的色谱分离性能

英文题名：POLY (STYRENE-CO-DIVINYLBENZENE) MONOLITHIC COLUMN FOR SEPARATION OF PROTEINS

作者：高利锋[1];徐环昕[1];刘坐镇[1];邬行彦[1]

机构：[1]华东理工大学生物反应器国家重点实验室,上海200237

年份：2006

卷号：22

期号：3

起止页码：261

中文期刊名：离子交换与吸附

外文期刊名：Ion Exchange and Adsorption

收录：CSTPCD;;EI(收录号：20063110034434);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：整体柱;聚(苯乙烯-二乙烯基苯);反相色谱;蛋白质分离;总分离效能指标K1

外文关键词：Monolithic column; Poly（styrene-co-divinylbenzene）; Reversed phase chromatography; Protein separation; Over-all resolution efficiency K1

摘要：苯乙烯(St)为单体,二乙烯基苯(DVB)为交联剂,在致孔剂甲苯和十二醇存在下,直接以Ф4.6×50mm色谱柱管为模具,同时在整个反应过程中通过氩气对反应混合物施加一个恒定的压力,通过原位聚合制备了聚(苯乙烯-二乙烯基苯)(PSD)型整体柱。在聚合过程中对反应混合物施加一定的压力,使制备的整体柱表面光滑无凹痕现象,并且使柱子的两头平整不凹陷,有利于提高柱效。本文对整体柱的孔结构及其对蛋白质的分离性能进行了研究。实验结果表明,整体柱内部含有大量类似渠道的大孔,孔径为2μm^3μm,在流速为5ml/min时,背压为7MPa。而且流速对色谱分离效率的影响小,流速从1ml/min增大到3ml/min时,牛血清白蛋白和溶菌酶这两种蛋白质的总分离效能指标K1的值基本保持不变,保持在3.35左右,说明可以通过提高流速实现蛋白质的快速分离。
A monolithic column of macroporous poly（styrene-co-divinylbenzcne） has been prepared by a free radical polymerization within the confines of a 50×4.6mm I.D. chromatographic stainless steel tube. During polymerization, pressure was applied to the polymerization solution using argon gas, thereby the monolithic polymeric column had a smooth surface with no dents on the surface, and both ends of the column were fiat and not sunken, improving the chromatographic resolution of the monolithic column. The pore structure of the monolithic column was characterized and was successfully used for the chromatographic separation of proteins. In the monolithic column there were macropores of 2μm～3μm. The back pressure of the monolithic column was 7MPa at a rate of 5mL/min. The chromatographic resolution of the monolithic column was basically independent of the flow rate. The value of over-all resolution efficiency of two test proteins i.e. BSA and Lysozyme was basically independent of the flow rate in the range of 1ml/min～3ml/min. Therefore, proteins can be separated at a high flow rate without the loss of resolution.