文献类型：期刊文献

英文题名：Production of Succinate from Acetate by Metabolically Engineered Escherichia coli

作者：Li, Yunjie[1,4];Huang, Bing[1];Wu, Hui[1];Li, Zhimin[1,2];Ye, Qin[1];Zhang, Y-H Percival[3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Virginia Tech, Dept Biol Syst Engn, 304 Seitz Hall, Blacksburg, VA 24061 USA;[4]Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West Seventh Ave, Tianjin 300308, Peoples R China

年份：2016

卷号：5

期号：11

起止页码：1299

外文期刊名：ACS SYNTHETIC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000388428100014)】；

基金：This study was supported by the National Science Foundation for Young Scientist of China (Grant No. 21406065), the Fundamental Research Funds for the Central Universities (Grant No. 222201313007 and No. 22A201514042), the National High Technology Research and Development Program of China (Grant No. 2012AA022104 and No. 2012AA021205), and the Foundation of Key Laboratory for Industrial Biocatalysis (Tsinghua University), Ministry of Education.

语种：英文

外文关键词：acetate; succinate; metabolic engineering; Escherichia coli; malic enzyme; citrate synthase

摘要：Acetate, a major component of industrial biological wastewater and of lignocellulosic biomass hydrolysate, could potentially be a less costly alternative carbon source. Here we engineered Escherichia coli MG1655 strain for succinate production from acetate as the sole carbon source. Strategies of metabolic engineering included the blockage of the TCA cycle, redirection of the gluconeogenesis pathway, and enhancement of the glyoxylate shunt. The engineered strain MG03 featuring the deletion of genes: succinate dehydrogenase (sdhAB), isocitrate lyase regulator (iclR), and malic enzymes (maeB) accumulated 6.86 mM of succinate in 72 h. MG03(pTrc99a-gltA) overexpressing citrate synthase (g1tA) accumulated 16.45 mM of succinate and the yield reached 0.46 mol/mol, about 92% of the maximum theoretical yield. Resting-cell was adopted for the conversion of acetate to succinate, and the highest concentration of succinate achieved 61.7 mM.