文献类型：期刊文献

中文题名：酶法合成头孢氨苄的反应-双水相萃取耦合过程

英文题名：ENZYMATIC SYNTHESIS OF CEPHALEXIN INTEGRATED WITH AQUEOUS TWO-PHASE SYSTEMS

作者：朱建航[1];魏东芝[1];曹学君[1];袁中一[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,生物化学研究所,上海200237;[2]中国科学院上海生物化学研究所,上海200231

年份：2003

卷号：54

期号：1

起止页码：95

中文期刊名：化工学报

外文期刊名：CIESC Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目 (No 2 98730 14 )~~

语种：中文

中文关键词：酶法合成;反应-双水相萃取;耦合过程;分配系数;头孢氨苄;青霉素酰化酶;抗生素

外文关键词：aqueous two-phase systems, integration of reaction and extraction, partition coefficient, cephalexin,penicillin G acylase

摘要：应用聚乙二醇 (PEG) /硫酸镁双水相体系 (ATPS)进行了酶法合成头孢氨苄的反应 双水相萃取的耦合过程研究 .通过考察头孢氨苄、苯甘氨酸甲酯和 7 氨基脱乙酰氧基头孢烷酸 ( 7 ADCA)在ATPS中的分配行为建立了一个含 2 0 % (质量 )PEG 40 0和 12 % (质量 )硫酸镁的ATPS ,在此体系中头孢氨苄的分配系数为 6 7,苯甘氨酸甲酯的分配系数为 1 5 ,7 ADCA的分配系数为 1 2 ,且青霉素酰化酶的分配系数小于 0 0 1.底物、产物以及催化剂的分配特性有利于构建双水相体系应用于酶法合成头孢氨苄的反应 双水相萃取的耦合过程 .对此双水相体系中青霉素酰化酶的稳定性研究表明催化剂亦适合此体系的构建 .进而将此体系应用于头孢氨苄酶法合成 ,产率为 60 %左右 ,相对水相体系 2 0
The potentiality of the conversion of 7-aminodeacetoxicephalosporanic acid(7-ADCA) to cephalexin using penicillin G acylase (PGA) (penicillin amidohydrolase, EC3.5.1.11) was studied in aqueous two-phase systems (ATPS).In a system consisting of 20%(mass) PEG 400 and 12%(mass) magnesium sulfate, the partition coefficient of PGA was less than 0.01, PGA almost completely partitioned into the magnesium sulfate-rich bottom phase. Meanwhile, the partition coefficient of cephalexin was 6.7, the partition coefficients of phenylglycine methyl ester (PGME) and 7-ADCA were 1.5 and 1.2, respectively. Then the stability of PGA in 20%(mass) PEG 400/15% (mass) magnesium sulfate ATPS was investigated and the results showed that PGA was suitable for the enzymatic synthesis of cephalexin in ATPS. When pure enzyme was used for bioconversion, a high yield of cephalexin (60%) was achieved form 75 mmol·L -1 7-ADCA and 75 mmol·L -1 PGME in the developed ATPS.