文献类型：期刊文献

英文题名：Emamectin benzoate induces ROS-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cells

作者：Luan, Shaorong[1];Yun, Xinming[2];Rao, Wenbing[2];Xiao, Ciying[3];Xu, Zhikang[2];Lang, Jialin[2];Huang, Qingchun[2]

机构：[1]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：273

起止页码：90

外文期刊名：CHEMICO-BIOLOGICAL INTERACTIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000406467500010)】；

基金：We gratefully acknowledge financial support from Shanghai Foundation of Science and Technology (Grant number 15431906800).

语种：英文

外文关键词：Emamectin benzoate; Cytotoxic action; ROS; DNA damage; Cell apoptosis

摘要：Emamectin benzoate (EMB), a novel macrocyclic lactone insecticide, possesses high efficacy and beneficial selective toxicity in agriculture, but so far the EMB-induced cytotoxic action in arthropod insect remains unclear. The present studies were carried out to characterize the property of EMB on the induction of reactive oxygen species (ROS)-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cell model. Following the exposure to EMB at 2.5, 5, 10 or 15 mu M, the cells changed to be round, suspended and aggregated, and the decline of cell proliferating ability and cell viability was positively related with the exposure time. Median inhibitory concentration ( IC50) of EMB on cell viability was 3.72 mM during 72 h exposure. Apoptosis was induced in 29.8% ( 24 h) and 39.5% ( 48 h) of the cells by EMB at 15 mM, showing chromatin condensation in nuclei. The content of ROS in the cells increased rapidly as the concentration of EMB increased, and the pre-incubation of the cells with vitamin E significantly reduced the ROS accumulation. In the treatment of 15 mM EMB, the migrated cell nucleus with DNA strand breaks appeared a teardrop, pear-shaped, or large fan-like tail, and 63.1% of gamma H2AX-positive cells contained more than four foci, accompanying with high expression level of caspase-3 in time-dependent manner, which consequently led to cell apoptotic death. These evidences in ROS-mediated DNA damage and cell apoptosis induced by EMB may be helpful for deep understanding the cytotoxic action of EMB based on cell model. (C) 2017 Elsevier B.V. All rights reserved.