文献类型：期刊文献

英文题名：Ultra-high signal-to-noise ratio near-infrared chemiluminescent probe for in vivo sensing singlet oxygen

作者：Zhao, Meiling[1,2];Lu, Yao[1,2];Zhang, Yutao[1,2];Xue, Haoyun[1,2];Guo, Zhiqian[1,2]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn,Frontiers Sci Ctr Materiobiol, Shanghai 200237, Peoples R China

年份：2025

卷号：36

期号：5

外文期刊名：CHINESE CHEMICAL LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001455798200001)】；

基金：This work was supported by National Natural Science Foundation of China (Nos. 32121005 , 22225805 , 22308101 , and 32394001) , Shanghai Science and Technology Innovation Action Plan (No. 23J21901600) , Innovation Program of Shanghai Municipal Education Commission, Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Munici-pal Education Commission , No. 2021 Sci & Tech 03-28) , the China Postdoctoral Science Foundation (No. 2021M701199) , Natural Science Foundation of Shanghai (No. 23ZR1416600) .

语种：英文

外文关键词：Fluorescent probe; Near-infrared; Chemiluminescent; Singlet oxygen; Bioimaging

摘要：Singlet oxygen (1 O2 ), as the primary reactive oxygen species in photodynamic therapy, can effectively induce excessive oxidative stress to ablate tumors and kill germs in clinical treatment. However, monitoring endogenous 1 O2 is greatly challenging due to its extremely short lifetime and high reactivity in biological condition. Herein, we report an ultra-high signal-to-ratio near-infrared chemiluminescent probe (DCMCy) for the precise detection of endogenous 1 O2 during photodynamic therapy (PDT). The methoxy moiety was removed from enolether unit in DCM-Cy to suppress the potential self-photooxidation reaction, thus greatly eliminating the photoinduced background signals during PDT. Additionally, the compact cyclobutane modification of DCM-Cy resulted in a significant 6-fold increase in cell permeability compared to conventional adamantane-dioxane probes. Therefore, our "step-by-step" strategy for DCM-Cy addressed the limitations of traditional chemiluminescent (CL) probes for 1 O2 , enabling effectively tracking of endogenous 1 O2 level changes in living cells, pathogenic bacteria and mice in PDT. (c) 2025 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.