文献类型：期刊文献

英文题名：Combinatorial strategies for production improvement of red pigments from Antarctic fungusGeomycessp.

作者：Huang, Hezhou[1];Ding, Lulu[1];Lu, Jian[1];Wang, Nengfei[2];Cai, Menghao[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Minist Nat Resources, Inst Oceanog 1, Qingdao, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai, Peoples R China

年份：2020

卷号：85

期号：10

起止页码：3061

外文期刊名：JOURNAL OF FOOD SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000566714600001)】；

基金：This work was supported by the National Key R&D Program of China (grant number 2018YFC1406706, 2018YFC1406700), Fundamental Research Funds for the Central Universities, China (grant number JKF012016019, 22221818014), and the 111 Project of China (grant number B18022). We thank Prof. Li Zhao, Department of Sanitary Microbiology, School of Public Health, Shandong University, for her contribution on HPLC analysis of citrinin content.

语种：英文

外文关键词：Antarctic fungus; ARTP; fermentation optimization; Geomycessp; medium development; natural red pigments

摘要：Natural red pigments have been widely used as food and cosmetics additives. However, due to toxic byproducts or allergen issues, it is still necessary to look for some other red pigment products. This study proposed combinatorial strategies to improve production of a new kind of red pigments from the fungusGeomycesWNF-15A, isolated from Antarctica. A high-production medium was developed by statistical experimental design, which was further simplified for industrial use by single-factor experiments. Strain breeding by atmospheric room temperature plasma mutagenesis generated a mutant,Geomycessp. WNF-15A-M210, which increased production of red pigments by 24.4% and shortened culture phase by 33.3% comparing with the wild-type. The production of red pigments by this mutant favored a weak alkaline condition but required only mild dissolved oxygen tension. Control of initial pH 8.5 (process pH around 7.5) increased red pigments production by 19% comparing with natural condition. Precursor and inhibitor addition experiments indicated that the red pigments were synthesized by polyketide pathway, and feeding 6 mmol/L precursor of sodium acetate by three aliquots at days 3 to 5 improved biosynthesis of red pigments by 27%. Finally, the developed culture process was verified in a 5-L stirred tank bioreactor. The red pigments production of the pH regulation group reached 1.11-fold of the control and 1.95-fold of the precursor regulation group, respectively. This study provides high-production strain, optimized medium, and bioprocess for the possible industrial production of AntarcticGeomycesred pigments in future. Practical Application AntarcticGeomycesred pigments showed high color value, nontoxic characteristic, and good water solubility. It holds potential for industrial use and is under development for food additive in China currently. This study provides an optional manufacturing process for this new kind of red pigments.