文献类型：期刊文献

中文题名：调节细胞通透性促进天蓝色链霉菌蓝色素生物合成

英文题名：REGULATION OF CELLULAR PERMEABILITY FOR IMPROVING BLUE PIGMENT BIOSYNTHSIS IN STREPTOMYCES COELICOLOR FERMENTATION

作者：张和春[1];范卫民[1];张元兴[1];王武[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]江南大学生物工程学院,无锡214036

年份：2002

卷号：8

期号：5

起止页码：540

中文期刊名：应用与环境生物学报

外文期刊名：Chinese Journal of Applied and Environmental Biology

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：细胞通透性;天蓝色链霉菌;蓝色素;生物合成;发酵

外文关键词：cellular permeability; blue pigment; Streptomyces coelicolor ; fermentation

摘要：在天蓝色链霉菌分批发酵中 ,36h时一次加入 2 0mg/L的青霉素 ,色素浓度和分泌率分别提高了 2 6 .2 %和39.2 % ;与一次性添加青霉素相比 ,分多次添加可使色素浓度再提高 9% ,但分泌率没有明显变化 .DMSO浓度为 6 g/L时 ,色素浓度和分泌率较对照分别提高了 2 0 .5 %和 5 0 % ;2～ 5 0 g/L的DMSO对菌体生长没有影响 .36h时加入 1g/L的吐温 80 ,色素浓度和分泌率分别提高了 16 .9%和 37.5 % .6 0h时加入 0 .2 g/L浓度的SDS ,色素浓度较对照基本相当 ,但分泌率却提高了 16 .1% .图 4表 1参
In the batch fermentation of Streptomyces coelicolor , blue pigment production increased by 26.2% and 39.2% when adding 20 mg/L penicillin by single and multi additive way, respectively, while there was no obvious influence to excretion ratio. It also demonstrated that blue pigment production and excretion ratio increased by 20.5% and 50% after supplemented with 6 g/L DMSO. 2～50 g/L DMSO had no effect on the growth of Streptomyces coelicolor . When adding 1 g/L Toween 80 at 36 h, blue pigment production and excretion ratio increased by 16.9% and 37.5%, respectively, while they increased by 6% and 16.1% if adding 0.2 g/L SDS at 60 h. Fig 4, Tab 1, Ref 7