文献类型：期刊文献

中文题名：An acid-triggered porphyrin-based block copolymer for enhanced photodynamic antibacterial efficacy

作者：Fan Yu[1];Chao Chen[1];Guoliang Yang[1];Zhengtong Ren[1];Hongliang Cao[1];Liangshun Zhang[1];Weian Zhang[1]

机构：[1]Shanghai Key Laboratory of Functional Materials Chemistry,East China University of Science and Technology,Shanghai 200237,China

年份：2021

卷号：64

期号：3

起止页码：459

中文期刊名：Science China Chemistry

外文期刊名：中国科学（化学英文版）

收录：Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：supported by the National Natural Science Foundation of China(21875063);the Science and Technology Commission of Shanghai Municipality for the Shanghai International Cooperation Program(19440710600)。

语种：英文

中文关键词：photodynamic therapy;antibacterial;porphyrin;aggregation caused quenching(ACQ);

摘要：Bacterial infection, especially multidrug-resistant(MDR) bacterial infection has threatened public health drastically. Here, we fabricate an "acid-triggered" nanoplatform for enhanced photodynamic antibacterial activity by reducing the aggregation of photosensitizers(PSs) in bacterial acidic microenvironment. Specifically, a functional amphiphilic block copolymer was first synthesized by using a pH-sensitive monomer, 2-(diisopropylamino) ethyl methacrylate(DPA) and porphyrin-based methacrylate(TPPC6MA) with poly(oligo(ethylene glycol) methyl ether methacrylate)(POEGMA) as the macromolecular chain transfer agent, and POEGMA-b-[PDPA-co-PTPPC6MA] block copolymer was further self-assembled into spherical nanoparticles(PDPA-TPP). PDPA-TPP nanoparticles possess an effective electrostatic adherence to negatively charged bacterial cell membrane, since they could rapidly achieve positive charge in acidic bacterial media. Meanwhile, the acid-triggered dissociation of PDPA-TPP nanoparticles could reduce the aggregation caused quenching(ACQ) of the photosensitizers, leading to around 5 folds increase of the singlet oxygen(1O2) quantum yield. In vitro results demonstrated that the "acid-triggered" PDPA-TPP nanoparticles could kill most of MDR S. aureus(Gram-positive) and MDR E. coli(Gram-negative) by enhanced photodynamic therapy, and they could resist wound infection and accelerate wound healing effectively in vivo. Furthermore, PDPA-TPP nanoparticles could well disperse the biofilm and almost kill all the biofilm-containing bacteria. Thus, by making use of the bacterial acidic microenvironment, this "acid-triggered" nanoplatform in situ will open a new path to solve the aggregation of photosensitizers for combating broad-spectrum drug-resistant bacterial infection.