文献类型：期刊文献

英文题名：Physicochemical properties, antioxidant and cardioprotective activities of polysaccharides from fermented Polygonatum kingianum with Lactobacillus species

作者：Zhang, Peng[1];Zhang, Hui[2];Wu, Menqi[2];Li, Yunyu[2];Xia, Wei[2];Zhu, Xuedong[1];Zhang, Wenqing[2]

机构：[1]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Large Scale Reactor Engn & Technol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2024

卷号：216

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20242116121851);WOS:【SCI-EXPANDED(收录号:WOS:001243442100001)】；

基金：This work was sponsored by "Science and Technology Innovation Action Plan" for Shanghai Municipal Science and Technology Support Project (Grant No. 22S21902800) . The authors thank the Yunnan Linyuan Spice Co., Ltd. (Yunnan, China) for providing the polygonati rhizome of Polygonatum kingianum .

语种：英文

外文关键词：Polygonatum kingianum; Lactobacillus; Physicochemical properties; Polysaccharides; Antioxidant activity; Cardioprotective activity

摘要：Polygonatum kingianum is usually used as a Chinese traditional homology of medicine and foods. P. kingianum polysaccharides exhibit various biological activities. To date, the processing of P. kingianum with Lactobacillus fermentation has been rarely explored. This study investigated the optimum fermentation conditions of P. kingianum using Lactobacillus plantarum and Lactobacillus rhamnosus by response surface methodology (RSM), followed by the extraction and characterization of fermented polysaccharides (namely MFP and SFP respectively), in contrast to non-fermented polysaccharide (NFP). Their changes in physicochemical properties, antioxidant, and cardioprotective activities were studied. High-performance size-exclusion chromatography analysis results demonstrated that a reduction in the proportion of the lower molecular weight fraction II (2.9 kD) of MFP and SFP. Compared to NFP, both MFP and SFP showed increased levels of galactose, galacturonic acid, and rhamnose, but decreased mannose, galactosamine, glucuronic acid, glucose, fucose, and fructose. Furthermore, the 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2 ' -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays revealed the higher antioxidant activities of MFP and SFP. Bioassay results showed that MFP and SFP possessed cardioprotective activities against hypoxia/reoxygenation injury in cardiomyocytes through the alleviation of oxidative stress and the inhibition of cell apoptosis. Collectively, these results could provide a reference for largescale production of polysaccharides form P. kingianum using Lactobacillus in medical and food industries.