文献类型：期刊文献

英文题名：Fabrication of PLGA/MWNTs composite electrospun fibrous scaffolds for improved myogenic differentiation of C2C12 cells

作者：Xu, Jiazhu[1];Xie, Ya[2];Zhang, Hongbo[2];Ye, Zhaoyang[1];Zhang, Wenjun[2,3]

机构：[1]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Mech & Power Engn, Complex & Intelligent Res Ctr, Shanghai 200237, Peoples R China;[3]Univ Saskatchewan, Dept Biomed Engn, Saskatoon, SK S7N 0W0, Canada

年份：2014

卷号：123

起止页码：907

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;EI(收录号：20145000321944);WOS:【SCI-EXPANDED(收录号:WOS:000347580500114)】；

基金：This work is in part supported by Chinese 1000 Talent's program. Financial supports from SHRF through a Phase I group project grant (to W. Z.) and National Natural Science Foundation of China 31000424(to Z. Y.) were also acknowledged.

语种：英文

外文关键词：Poly(lactic-co-glycolic acid); Carbon nanotubes; Electrospinning; Electrical conductivity; C2C12 cells; Skeletal muscle tissue engineering

摘要：Electrically conducting scaffolds have attracted tremendous attention in skeletal muscle tissue engineering. In this paper, poly(lactic-co-glycolic acid) (PLGA)/multi-wall carbon nanotubes (MWNTs) composite fibrous scaffolds were fabricated using the electrospinning technique. The physical properties of the composite fibers were characterized and proliferation and differentiation of C2C12 cells on these scaffolds were examined. It was found that the addition of MWNTs modulated the physical properties of PLGA fibers including morphology, fiber diameter, degradation, tensile strength and electrical conductivity, depending on the amount of MWNTs. These fibrous scaffolds were cytocompatible and supported the proliferation of C2C12 cells. Importantly, C2C12 cells showed more mature myotube formation on PLGA/MWNTs composite fibrous scaffolds compared to PLGA scaffolds. These results indicate that PLGA/MWNTs composite electrospun fibers have great potential in skeletal muscle tissue engineering. (C) 2014 Elsevier B.V. All rights reserved.