文献类型：期刊文献

英文题名：Delivery of Paclitaxel Using PEGylated Graphene Oxide as a Nanocarrier

作者：Xu, Zhiyuan[1,2];Zhu, Shaojia[2];Wang, Mingwei[3];Li, Yongjun[1];Shi, Ping[2];Huang, Xiaoyu[1]

机构：[1]Chinese Acad Sci, Shanghai Inst Organ Chem, Key Lab Synthet & Self Assembly Chem Organ Funct, Shanghai 200032, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Fudan Univ, Shanghai Canc Ctr, Dept Nucl Med, Shanghai 200032, Peoples R China

年份：2015

卷号：7

期号：2

起止页码：1355

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20150500463559);WOS:【SCI-EXPANDED(收录号:WOS:000348332700039)】；

基金：The authors are thankful for financial support from the National Natural Science Foundation of China (21204098, 31100549, and 11275050), Shanghai Scientific and Technological Innovation Project (11 nm0501100, 11ZR1445900, 14520720100, and 14520720700), State Key Laboratory of Bioreactor Engineering (2060204), and Fundamental Research Funds for the Central Universities (222201313010). We thank Prof. Guowei Wang (Fudan Univeristy) for assistance in synthesizing 6-armed PEG.

语种：英文

外文关键词：paclitaxel; graphene oxide; PEG; drug delivery

摘要：Paclitaxel (PTX) is an extensively used potent chemotherapy drug; however, low water solubility, poor bioavailability, and emergence of drug resistance in patients limited its biological application. In this report, we proposed a new drug delivery system for cancer therapy based on graphene oxide (GO), a novel 2D nanomaterial obtained from the oxidation of natural graphite, to improve the utilization rate of PTX. PTX was first connected to biocompatible 6-armed poly(ethylene glycol), followed by covalent introduction into the surface of GO sheets via a facile amidation process under mild conditions, affording the drug delivery system, GO-PEG-PTX (size 50200 nm). GO-PEG nanosized carrier could quickly enter into human lung cancer A549 and human breast cancer MCF-7 cells verified by inverted fluorescence microscope using fluorescein isothiocyanate as probe. This nanocarrier was nontoxic to A549 and MCF-7 cells without linking with PTX. Nevertheless, GO-PEG-PTX showed remarkably high cytotoxicity to A549 and MCF-7 cells in a broad range of concentration of PTX and time compared to free PTX. This kind of nanoscale drug delivery system based on PEGylated GO may find widespread application in biomedicine.