文献类型：期刊文献

中文题名：嗜碱杆菌甘露聚糖酶环境适应性及其对瓜尔胶的降解作用

英文题名：Environmental Adaptability of Alkalophilic Mannanase and Its Degradation of Guar Gum

作者：王丽仙[1];李良川[2];刘彝[2];杨世忠[1,3];刘金峰[1,3]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]中国石油冀东油田钻采工艺研究院,河北唐山063000;[3]生物采油教育部工程研究中心,上海200237

年份：2018

卷号：44

期号：5

起止页码：661

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

语种：中文

中文关键词：甘露聚糖酶;酶降解;瓜尔胶;黏度;降解产物

外文关键词：mannanases;enzymatic degradation;guar gum;viscosity;degradation products

摘要：针对油田压裂生产中普遍使用的瓜尔胶,采用凝胶渗透色谱、电喷雾质谱等方法,结合黏度、还原糖等分析,研究了甘露聚糖酶Man-14在不同温度和pH条件下的适应性及降解特性。结果显示,在温度55～70℃、pH 7.0～9.5范围内,0.49U的Man-14能够在20min内将瓜尔胶溶液黏度从400mPa·s降至5mPa·s以下;与常用氧化剂过硫酸铵(APS)相比,Man-14降解产物分子量更小,残渣量更少,降解速率更快。
The application of guar gum in oilfield exploitation,especially in fracturing fluids,has gained increasing attention.As it is demanded,the guar gum in fracturing fluid must be subsequently degraded after being injected into the fracture produced in the process of well stimulation,and thus enable the oil to flow to the wellbore.For this purpose,the fast and effective degraders of guar gum are anticipated,and the apparent viscosity and degradation products are considered to be very important factors that influence the effect of hydraulic fracturing.Mannanase is an effective degrader of guar gum and shows great potential in oil reservoir fracturing due to its high activity and environment friendly nature.However,till now,the knowledge on the performance of mannanase to degrade guar gum under extreme temperature and pH conditions is limited.In this study,mannanase-catalyzed guar gum degradation at different temperatures and pH values was analyzed mainly by combination of GPC and ESI-MS.Comprehensive data with respect to guar gum degradation were obtained including the composition,molecular weight distribution,and reducing sugar content of degradation products as well as the reduction of viscosity of guar gum solution after degradation.The results revealed that mannanase Man-14 showed high guar gum degradation activity under a wide range of temperature and pH.The viscosity of guar gum solution was reduced to a value below 5 mPa·s in 20 min by 0.49 U Man-14 at the temperature between 55℃to 70℃and pH between 7.0 to 9.5.Furthermore,the molecular mass of the degradation products produced by Man-14 were much smaller than those by APS even with the same post-degradation viscosity of guar gum solution.The molecular mass of guar was reduced by enzymatic degradation from the initial 2×10 6 Du to 3 800 Du within 3 h at the temperature 35℃or 70℃,and the oligosaccharides with polymerization degree of 2-5 was detected by ESI-MS analysis.This study provides more information on guar gum degradation with mannanase and is helpful for its applications,especially in oil reservoir fracturing for breaking guar gum.