文献类型：期刊文献

英文题名：Coordinate regulation of carbohydrate metabolism and virulence by PtsH in pathogen Edwardsiella piscicida

作者：Mao, Qiaoqiao[1];Jiang, Jihao[1];Wu, Xiao[1];Xu, Rongjing[2];Ma, Yue[1,3];Zhang, Yuanxing[3,4];Shao, Shuai[1,3];Wang, Qiyao[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Yantai Tianyuan Aquat Co Ltd, Yantai, Shandong, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China

年份：2022

卷号：106

期号：5-6

起止页码：2063

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20220911721013);WOS:【SCI-EXPANDED(收录号:WOS:000761867000003)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (32130108, 32002436), China Agriculture Research System of MOF and MARA (CARS-47), and the Fundamental Research Funds for the Central Universities (22221062017019).

语种：英文

外文关键词：Edwardsiella piscicida; Mannose; Phosphotransferase system; Type III secretion system (T3SS); Virulence

摘要：Carbohydrate metabolism of bacterial pathogens conducts crucial roles in regulating pathogenesis but the molecular mechanisms by which metabolisms and virulence are been modulated and coordinated remain to be illuminated. Here, we investigated in this regard Edwardsiella piscicida, a notorious zoonotic pathogen previously named E. tarda that could ferment very few PTS sugars including glucose, fructose, mannose, N-acetylglucosamine, and N-acetylgalactosamine. We systematically characterized the roles of each of the predicted 23 components of phosphotransferase system (PTS) with the respective in-frame deletion mutants and defined medium containing specific PTS sugar. In addition, PtsH was identified as the crucial PTS component potentiating the utilization of all the tested PTS sugars. Intriguingly, we also found that PtsH while not Fpr was involved in T3SS gene expression and was essential for the pathogenesis of E. piscicida. To corroborate this, His15 and Ser46, the two established PtsH residues involved in phosphorylation cascade, showed redundant roles in regulating T3SS yields. Moreover, PtsH was shown to facilitate mannose uptake and transform it into mannose-6-phosphate, an allosteric substrate established to activate EvrA to augment bacterial virulence. Collectively, our observations provide new insights into the roles of PTS reciprocally regulating carbohydrate metabolism and virulence gene expression. Key points PTS components' roles for sugar uptake are systematically determined in Edwardsiella piscicida. PtsH is involved in saccharides uptake and in the regulation of E. piscicida's T3SS expression. PtsH phosphorylation at His15 and Ser46 is essential for the T3SS expression and virulence.