文献类型：期刊文献

英文题名：Oxidative stress contributes to silica nanoparticle-induced cytotoxicity in human embryonic kidney cells

作者：Wang, Fen[1];Gao, Feng[1];Lan, Minbo[2];Yuan, Huihui[2];Huang, Yongping[3];Liu, Jianwen[3]

机构：[1]E China Univ Sci & Technol, Dept Pharmaceut, Sch Pharm, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Testing Informat Anal Ctr, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Dept Pharmacol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2009

卷号：23

期号：5

起止页码：808

外文期刊名：TOXICOLOGY IN VITRO

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000268345500007)】；

基金：Financial support from the Shanghai nanotechnology Leading Academic Discipline projects (project no. 0752nm025) is gratefully acknowledged.

语种：英文

外文关键词：Silica nanoparticles; Cytotoxicity; Oxidative stress

摘要：In order to elucidate the nanoparticle-induced cytotoxicity and its mechanism, the effects of 20 and 50 nm silica nanoparticles on cultured human embryonic kidney (HEK293) cells were investigated. Cell viability, mitochondrial function, cell morphology, reactive oxygen species (ROS), glutathione (GSH), thiobarbituric acid reactive substance (TBARS), cell cycle and apoptosis were assessed under control and silica exposed conditions. Exposure to 20 or 50 nm SiO2 nanoparticles at dosage levels between 20 and 100 mu g/ml decreased cell viability in a dose-dependent manner. Median lethal dose (LD50) of 24 h exposure was 80.2 +/- 6.4 and 140.3 +/- 8.6 mu g/ml for 20 and 50 nm SiO2 nanoparticles, respectively. Morphological examination revealed cell shrinkage and nuclear condensation after SiO2 nanoparticle exposure. Increase in intracellular ROS level and reduction in GSH content were also observed in SiO2 nanoparticle-exposed HEK293 cells. Increase in the amount of TBARS suggested an elevated level of lipid peroxidation. Flow cytometric analysis showed that SiO2 nanoparticles can cause G2/M phase arrest and apoptotic sub-G1 population increase in a dose-dependent manner. In summary, exposure to SiO2 nanoparticles resulted in a dose-dependent cytotoxicity in cultured HEK293 cells that was associated with increased oxidative stress. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.