文献类型：期刊文献

英文题名：A Bacterial Pathogen Senses Host Mannose to Coordinate Virulence

作者：Wei, Lifan[1];Qiao, Haoxian[1];Sit, Brandon[4,5];Yin, Kaiyu[1];Yang, Guanhua[1];Ma, Ruiqing[1];Ma, Jiabao[1];Yang, Chun[7];Yao, Jun[8];Ma, Yue[1,3];Xiao, Jingfan[1];Liu, Xiaohong[1,3];Zhang, Yuanxing[1,3];Waldor, Matthew K.[1,4,5,6];Wang, Qiyao[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Shandong, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Brigham & Womens Hosp, Div Infect Dis, Boston, MA 02115 USA;[5]Harvard Med Sch, Boston, MA 02115 USA;[6]Howard Hughes Med Inst, Boston, MA 02115 USA;[7]Fudan Univ, Sch Life Sci, Dept Physiol & Biophys, State Key Lab Genet Engn, Shanghai 200433, Peoples R China;[8]Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

年份：2019

卷号：20

起止页码：310

外文期刊名：ISCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000493388000024)】；

基金：This work was supported by grants from the National Key Research and Development Program of China (2018YFD0900504 to Q.W.), National Natural Science Foundation of China (31430090 to Y.Z., 31602200 to X.L.), Ministry of Agriculture of China (CARS-47), and the Science and Technology Commission of Shandong and Shanghai Municipality (2017CXGC0103 and 17391902000). B.S. is supported by the Natural Sciences and Engineering Council of Canada (PGSD3-487259-2016). M.K.W. is supported by the Howard Hughes Medical Institute (HHMI) (CC30430) and the National Institutes of Health (RO1-AI-042347).

语种：英文

摘要：Bacterial pathogens are thought to activate expression of virulence genes upon detection of host-associated cues, but identification of the nature of such signals has proved difficult. We generated a genome-scale defined transposon mutant library in Edwardsiella piscicida, an important fish pathogen, to quantify the fitness of insertion mutants for intracellular growth in macrophages and in turbot (Scophthalmus maximus). These screens identified EvrA, a transcription activator that induces expression of esrB, a key virulence regulator. EvrA is directly bound and activated by mannose-6-phosphate (man-6P) derived from actively imported mannose. Mutants lacking EvrA or expressing an EvrA unable to bind man-6P were similarly attenuated in turbot. Exogenously added mannose promoted E. piscicida virulence, and high levels of mannose were detected in fish tissue. Together, these observations reveal that binding of a host-derived sugar to a transcription factor can facilitate pathogen sensing of the host environment and trigger virulence programs.