文献类型：期刊文献

英文题名：From wheat straw to soluble yeast extract: enhanced mycoprotein production by adaptively evolved Trichosporon cutaneum and fermentation pH shifting

作者：Qu, Chaolong[1];Ren, Dayu[1];Liu, Qi[1];Zhang, Bin[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：48

期号：11

起止页码：1897

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20253318965880);WOS:【SCI-EXPANDED(收录号:WOS:001545498300001)】；

基金：This research was supported by the National Key R&D Program of China (2023YFA0914400), the National Natural Science Foundation of China (32301269), the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900), and the Key Technology R&D Program of the Science and Technology Commission of Shanghai Municipality (25HC2820200).

语种：英文

外文关键词：Single cell protein; Lignocellulose; Adaptive evolution; pH shifting; Yeast extract

摘要：Single-cell protein (SCP) produced by yeast using low-cost agricultural wastes shows great potential as an alternative protein source for animal and human nutrition. In this study, we developed an adaptive evolution method coupled with centrifugal fractionation and pH shifting to enhance SCP production by Trichosporon cutaneum from wheat straw. During the adaptive evolution, the culture pH was shifted from 5.0 to 7.0, which is more favorable for SCP accumulation of T. cutaneum. The finally obtained T. cutaneum CL160 exhibited a 109.2% increase in SCP content compared to the parental strain. The DCW and SCP titer of T. cutaneum CL160 reached 48.6 +/- 1.5 g/L and 14.2 +/- 1.1 g/L using wheat straw clarified hydrolysate by batch fermentation. Fed-batch fermentation using wheat straw-derived syrup further improved DCW and SCP titer to 124.2 g/L and 32.6 g/L. Further attempts were performed to prepare soluble yeast extract from lignocellulose-derived SCP by cell autolysis. This yeast extract served as an effective nitrogen source for lactic acid fermentation by Pediococcus acidilactici, achieving 83.2 +/- 1.1 g/L lactic acid titer and 45 x 109/mL CFU value, comparable to commercial yeast extract. This study demonstrates the conversion of waste lignocellulosic feedstocks into sustainable SCP and soluble yeast extract, presenting an innovative strategy for the valorization of non-food lignocellulosic feedstocks.