文献类型：期刊文献

英文题名：Combination of integrated expanded bed adsorption chromatography and countercurrent chromatography for the direct extraction and purification of pseudohypericin and hypericin from St. John's wort (Hypericum perforatum L.)

作者：Cai, Fanfan[1];Li, Yang[1];Zhang, Min[2,3];Zhang, Hongyang[1];Wang, Yuerong[1];Hu, Ping[1,4]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr TCM, Shanghai 200237, Peoples R China;[4]Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau, Peoples R China

年份：2015

卷号：38

期号：15

起止页码：2588

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20152500944287);WOS:【SCI-EXPANDED(收录号:WOS:000358443600004)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Nos. 81273481 and 81202493), the Fundamental Research Funds from the Central Universities, and National Science and Technology Major Project for Significant New Drugs Development (No. 2013ZX09507005).

语种：英文

外文关键词：Countercurrent chromatography; Expanded bed adsorption chromatography; Hypericin; Pseudohypericin; St. John's wort

摘要：St. John's wort has attracted particular attention because of its beneficial effects as an antidepressant, antiviral, and anticancer agent. A method for the combination of integrated expanded bed adsorption chromatography and countercurrent chromatography for the simultaneous extraction and purification of pseudohypericin and hypericin from the herb is presented in this paper. Firstly, the constituents were extracted and directly adsorbed by expanded bed adsorption chromatography under optimal conditions. The stepwise elution was then performed by expanded bed adsorption chromatography that enriched the targets with higher purities and recoveries compared to other methods. Secondly, the eluent fractions from expanded bed adsorption chromatography were further separated by two-step high-speed countercurrent chromatography. A two-step high-speed countercurrent chromatography method with a biphasic solvent system composed of n-hexane/ethyl acetate/methanol/water with a volume ratio of 1:2:1:2 was performed by stepwise changing the flow rate of the mobile phase. Consequently, 5.6 mg of pseudohypericin and 2.2 mg of hypericin with purities of 95.5 and 95.0%, respectively, were successfully obtained from 40 mg of crude sample.