文献类型：期刊文献

英文题名：Photostable red turn-on fluorescent diketopyrrolopyrrole chemodosimeters for the detection of cysteine in living cells

作者：Qu, Weisong[1,2];Yang, Lin[1,2];Hang, Yandi[1,2];Zhang, Xiao[1,2];Qu, Yi[3];Hua, Jianli[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]Shanghai Univ Engn Sci, Coll Chem & Chem Engn, Shanghai 201620, Peoples R China

年份：2015

卷号：211

起止页码：275

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20150700520108);WOS:【SCI-EXPANDED(收录号:WOS:000350003700036)】；

基金：This work was supported by the National Basic Research 973 Program (2013CB733700), NSFC/China (21421004, 21372082, 91233207, 21404068 and 21172073).

语种：英文

外文关键词：Fluorescent chemodosimeter; Cysteins; Diketopyrrolopyrrole; Photostability; Cell imaging

摘要：optical monitoring of thiols such as glutathione (GSH), cysteine (Cys) and homocysteine (Hcy) is of significant interest due to their crucial roles in maintaining the biological redox homeostasis through the equilibrium of free thiols and oxidized disulfides in biological systems. In this work, a molecular chemodosimeter DPP-DNBS with a highly photostable diketopyrrolopyrrole (DPP) fluorophore connected to a 2,4-dinitrobenzenesulfonyl (DNBS) electron-withdrawing group for selective detection of biothiols was effectively synthesized. The specific reaction between biological thiols and DPP-DNBS gives rise to an obvious fluorescence intensity enhancement at 615 nm which is induced by the cleavage of O-S bond liberates DPP fluorophore. High sensitivity and selectivity for pathless over other amino acids were also obtained. Moreover, via turn-on signal toward cysteine in Hela cells, DPP-DNBS was also employed in cellular detection of cysteine and realized discrimination of cysteine from homocysteine and glutathionein Hela cells. (C) 2015 Elsevier B.V. All rights reserved.