文献类型：期刊文献

英文题名：Study of double-bonded carboxymethyl chitosan/cysteamine-modified chondroitin sulfate composite dressing for hemostatic application

作者：Wang, Shumin[1];Ji, Xiaoguo[1];Chen, Shumin[1];Zhang, Chunyue[1,3];Wang, Yibing[1,3];Lin, Haodong[2];Zhao, Liming[1,3]

机构：[1]East China Univ Sci & Technol, Res & Dev Ctr Separat & Extract Technol Fermentat, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Orthoped Surg, Sch Med, Shanghai, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol SC, Shanghai 200237, Peoples R China

年份：2022

卷号：162

外文期刊名：EUROPEAN POLYMER JOURNAL

收录：;EI(收录号：20214711208873);WOS:【SCI-EXPANDED(收录号:WOS:000742685700004)】；

基金：This research was financially supported by the National Key R&D Program of China (2019YFD0901805), the 111 Project (B18022) and the Fundamental Research Funds for the Central Universities (22221818014).

语种：英文

外文关键词：Carboxymethyl chitosan; Chondroitin sulfate; Hydrogel; Freeze-dried dressing; Rapid hemostasis

摘要：Stanching severe traumatic bleeding requires hemostatic materials that can provide rapid and efficient hemostasis. However, most currently available hemostatic agents exhibit low efficiency and poor biocompatibility. In this study, a novel carboxymethyl chitosan modified with methacrylic anhydride (double-bonded carboxymethyl chitosan)/cysteamine-modified chondroitin sulfate (Me-CMC/CSS) rapid hemostatic dressing was developed using thiol-ene click chemistry. The freeze-dried Me-CMC/CSS dressings absorbed water rapidly and transformed into soft hydrogels that could seal wounds, achieving good hemostasis. The relative blood clotting index of the optimal Me-CMC/CSS dressing was decreased by 53% (p < 0.001) and 29% compared with a pure Me-CMC dressing and a commercial hemostatic gelatin sponge, respectively. In a rat liver hemorrhage model, the MeCMC/CSS dressing stopped bleeding rapidly, and the total blood loss was significantly decreased by 92% (p < 0.0001) compared with the untreated group and was lower than that with gelatin sponge treatment. The MeCMC/CSS dressing also exhibited a higher ability to adhere to platelets than the gelatin sponge. Furthermore, the Me-CMC/CSS dressing exhibited good hemocompatibility and cytocompatibility. The results indicate that the composite dressings can be potentially applied as rapid hemostatic materials.