文献类型：期刊文献

英文题名：Attomolar Ultrasensitive MicroRNA Detection by DNA-Scaffolded Silver-Nanocluster Probe Based on Isothermal Amplification

作者：Liu, Yu-Qiang[1];Zhang, Min[1];Yin, Bin-Cheng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：84

期号：12

起止页码：5165

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20122615154093);WOS:【SCI-EXPANDED(收录号:WOS:000305320500001)】；

基金：This work was financially supported by Grant NSF21075040, the Shanghai Fund (Grants 11 nm0502500 and 11XD1401900), and the Fundamental Research Funds for the Central Universities. We are also grateful to Dr. Wei-Wei Zhang and Prof. Hai-Bo Cai for kindly assisting in the cellbased assays and the support from Hitachi, Ltd.

语种：英文

外文关键词：Isotherms - Silver - Clinical research - RNA - Cost effectiveness - Diagnosis

摘要：MicroRNAs (miRNAs) play vital roles in a plethora of biological and cellular processes. The levels of miRNAs can be useful biomarkers for cellular events or disease diagnosis, thus the method for sensitive and selective detection of miRNAs is imperative to miRNA discovery, study, and clinical diagnosis. Here we develop a novel method to quantify miRNA expression levels as low as attomolar sensitivity by target-assisted isothermal exponential amplification coupled with fluorescent DNA-scaffolded AgNCs and demonstrated its feasibility in the application of detecting miRNA in real samples. The method reveals superior sensitivity with a detection limit of miRNA of 2 aM synthetic spike-in target miRNA under pure conditions (approximately 15 copies of a miRNA molecule in a volume of 10 mu L) and can detect at least a 10 aM spike-in target miRNA in cell lysates. The method also shows the high selectivity for discriminating differences between miRNA family members, thus providing a promising alternative to standard approaches for quantitative detection of miRNA. This simple and cost-effective strategy has a potential of becoming the major tool for simultaneous quantitative analysis of multiple miRNAs (biomarkers) in tissues or cells and supplies valuable information for biomedical research and clinical early diagnosis.