文献类型：期刊文献

英文题名：Characterization and Development of Salt-Resistant Aspergillus oryzae for Marine-Derived Enzyme Expression

作者：Han, Bei[1];Shao, Qianqian[1];Zhang, Yilun[1];Liu, Qi[1];Cai, Menghao[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2025

卷号：197

期号：11

起止页码：7258

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20253719149573);WOS:【SCI-EXPANDED(收录号:WOS:001566802100001)】；

基金：This study was supported by the National Key R&D Program of China (2022YFC2805102), National Natural Science Foundation of China (32201206), China Postdoctoral Science Foundation (2022M711146), and the 111 project (B18022).

语种：英文

外文关键词：Marine enzyme; A. oryzae; Transcriptomic analysis; Heterologous expression; Alginate lyase

摘要：Marine-derived enzymes often show distinct physiological properties and great potential for industrial use. Salt ions may improve the stability and expression efficiency of marine enzymes, which requires salt-resistant host based expression platform. Aspergillus oryzae of good protein expression and secretion was evaluated and explored for this purpose. Growth, sporulation, secreted extracellular proteins and transcriptome-based metabolic characteristics under artificial seawater condition were analyzed. Also, genetic manipulation system was developed and compared in various strains. Finally, A. oryzae AS 3.487 was recognized as a dominant chassis host. Efficient constitutive and artificial seawater-inducible promoters were screened from the transcriptomic data, which were further evaluated using marine alginate lyase AlgI as the reporter. Medium and condition optimizations improved enzyme production subsequently. Multicopy expression strategy further achieved a maximum enzyme production of 797 U/mL in flask culture. This study provides new references for the heterologous expression of marine-derived enzymes under high-salinity culture conditions.