文献类型：期刊文献

英文题名：Construction of a two-dimensional magnetic solid phase extraction microfluidic chip/tandem mass spectrometry platform for the determination of cytarabine in plasma

作者：Sun, Yangkun[1];Han, Yu[1];Zhao, Linghao[1];Xiang, Yuanzheng[1];Xiong, Wenjing[1];Zhang, Hongyang[1];Zhang, Min[2];Ning, Fanghong[3];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2024

卷号：207

外文期刊名：MICROCHEMICAL JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001357086200001)】；

基金：This research was funded by the National Natural Science Founda-tion of China (Grant number 81973285) .

语种：英文

外文关键词：Microfluidic chip-mass spectrometry; Two-dimensional solid phase extraction chip; Magnetic solid phase extractant; Therapeutic drug monitoring

摘要：A novel concept of two-dimensional magnetic solid phase extraction microfluidic chip (2D-MSPE-chip) was proposed, in which two or even more different separation mechanisms of solid phase extraction units were designed and constructed on microfluidic chips in series. The synthesized Fe3O4-UiO-66-C18 was used in the first dimension to retain protein and phospholipid through hydrophobic effect and Zr-O-P coordination, which could almost eliminate the interference of the matrix effect on the mass spectrum. The second dimensional material utilized Fe3O4-UiO-66-NH2 to enable cytarabine enrichment via it-it and hydrogen bonding. The parameters of 2D MSPE-Chip/tandem MS method such as desorption solvent (5 % acetic acid acetonitrile), loading solvent (20 % acetonitrile), and flow rate for extraction and desorption (10 mu L/min) were select. The LOD of the established method for cytarabine in plasma was 0.13 ng/mL, and the linear range was between 2.0 ng/mL and 1000 ng/mL with correlation coefficient (R2 = 0.9991). The matrix effect was -5.0 %. The recovery rate of real samples ranged from 95.5 % to 104.8 %, with RSD <= 5.2 %. The chip design was simple and efficient and could be reused, thereby decreasing analysis expenses. It held considerable potential for application in rapid clinical diagnostics and precision medicine.