文献类型：期刊文献

英文题名：Enhanced Cell Osteogenesis and Osteoimmunology Regulated by Piezoelectric Biomaterials with Controllable Surface Potential and Charges

作者：Mao, Lijie[1,2];Bai, Long[1,2];Wang, Xinqing[1,2];Chen, Xiaolei[1,2];Zhang, Dong[1,2];Chen, Fangping[1,2];Liu, Changsheng[1,2]

机构：[1]East China Univ Sci & Technol, Minist Educ, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2022

卷号：14

期号：39

起止页码：44111

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20224112850233);WOS:【SCI-EXPANDED(收录号:WOS:000864064400001)】；

基金：This investigation was supported by the National Natural Science Foundation of China (Nos. 32171342 and 51772100), Shanghai Science and Technology Agriculture Project (No. 202002080002F01474), National Key Research and Development Program of China (No. 2016YFC1102900), Shanghai Pujiang Program (16PJD015), and Frontiers Science Center (JKVD1211002).

语种：英文

外文关键词：piezoelectric; surface charges; osteogenesis; osteoimmunology; bone regeneration

摘要：Bone regeneration is a well-orchestrated process involving electrical, biochemical, and mechanical multiple physiological cues. Electrical signals play a vital role in the process of bone repair. The endogenous potential will spontaneously form on defect sites, guide the cell behaviors, and mediate bone healing when the bone fracture occurs. However, the mechanism on how the surface charges of implant potentially guides osteogenesis and osteoimmunology has not been clearly revealed yet. In this study, piezoelectric BaTiO3/beta-TCP (BTCP) ceramics are prepared by two-step sintering, and different surface charges are established by polarization. In addition, the cell osteogenesis and osteoimmunology of BMSCs and RAW264.7 on different surface charges were explored. The results showed that the piezoelectric constant d33 of BTCP was controllable by adjusting the sintering temperature and rate. The polarized BTCP with a negative surface charge (BTCP-) promoted protein adsorption and BMSC extracellular Ca2+ influx. The attachment, spreading, migration, and osteogenic differentiation of BMSCs were enhanced on BTCP-. Additionally, the polarized BTCP ceramics with a positive surface charge (BTCP+) significantly inhibited M1 polarization of macrophages, affecting the expression of the M1 marker in macrophages and changing secretion of proinflammatory cytokines. It in turn enhanced osteogenic differentiation of BMSCs, suggesting that positive surface charges could modulate the bone immunoregulatory properties and shift the immune microenvironment to one that favored osteogenesis. The result provides an alternative method of synergistically modulating cellular immunity and the osteogenesis function and enhancing the bone regeneration by fabricating piezoelectric biomaterials with electrical signals.