文献类型：期刊文献

英文题名：Stabilization and Induction of Oligonucleotide i-Motif Structure via Graphene Quantum Dots

作者：Chen, Xin[1];Zhou, Xuejiao[2];Han, Ting[2];Wu, Jiaying[1];Zhang, Jingyan[1];Guo, Shouwu[2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Sch Pharm, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Key Lab Thin Film & Microfabricat, Minist Educ, Res Inst Micro Nano Sci & Technol, Shanghai 200240, Peoples R China

年份：2013

卷号：7

期号：1

起止页码：531

外文期刊名：ACS NANO

收录：;EI(收录号：20130515964705);WOS:【SCI-EXPANDED(收录号:WOS:000314082800057)】；

基金：This work was financially supported by the National Science Foundation of China (Nos. 91123011, 90923041, 31070742), the State Key Laboratory of Bioreactor Engineering (No. 2060204), 111 Project (No. B07023), the Shanghai Committee of Science and Technology (No. 11DZ2260600), and National "973 Program" (No. 2010CB933900). Thanks to Lei Chen and Prof. Yun Tang for their help in generating the 3D structure in Scheme 1.

语种：英文

外文关键词：graphene quantum dots; i-motif; stabilization; induction; end-stacking

摘要：DNA i-motif structures have been found in telomeric, centromeric DNA and many in the promoter region of oncogenes; thus they might be attractive targets for gene-regulation processes and anticancer therapeutics. We demonstrate in this work that i-motif structures can be stabilized by graphene quantum dots (GQDs) under acidic conditions, and more importantly GODs can promote the formation of the i-motif structure under alkaline or physiological conditions. We Illustrate that the GQDs stabilize the i-motif structure through end-stacking of the bases at its loop regions, thus reducing its solvent-accessible area. Under physiological or alkaline conditions, the end-stacking of GQDs on the unfolded structure shifts the equilibrium between the I-motif and unfolded structure toward the i-motif structure, thus promoting its formation. The possibility of fine-tuning the stability of the I-motif and Inducing its formation would make GQDs useful in gene regulation and oligonucleotide-based therapeutics.