文献类型：期刊文献

英文题名：Newly Identified Thermostable Esterase from Sulfobacillus acidophilus: Properties and Performance in Phthalate Ester Degradation

作者：Zhang, Xiao-Yan[1];Fan, Xiang[1];Qiu, Yong-Jun[1,2];Li, Cheng-Yuan[1];Xing, Shuai[1];Zheng, Yi-Tao[1];Xu, Jian-He[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2014

卷号：80

期号：22

起止页码：6870

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;EI(收录号：20144500153716);WOS:【SCI-EXPANDED(收录号:WOS:000344161700003)】；

基金：This work was financially supported by the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology.

语种：英文

外文关键词：Escherichia coli - Esters - Hydrolysis

摘要：EstS1, a newly identified thermostable esterase from Sulfobacillus acidophilus DSM10332, was heterologously expressed in Escherichia coli and shown to enzymatically degrade phthalate esters (PAEs) to their corresponding monoalkyl PAEs. The optimal pH and temperature of the esterase were found to be 8.0 and 70 degrees C, respectively. The half-life of EstS1 at 60 degrees C was 15 h, indicating that the enzyme had good thermostability. The specificity constant (k(cat)/K-m) of the enzyme for p-nitrophenyl butyrate was as high as 6,770 mM(-1) s(-1). The potential value of EstS1 was demonstrated by its ability to effectively hydrolyze 35 to 82% of PAEs (10 mM) within 2 min at 37 degrees C, with all substrates being completely degraded within 24 h. At 60 degrees C, the time required for complete hydrolysis of most PAEs was reduced by half. To our knowledge, this enzyme is a new esterase identified from thermophiles that is able to degrade various PAEs at high temperatures.