文献类型：期刊文献

英文题名：Biological Sentinel: An Efficient Engineered Bacterial System for Monitoring and Tracing Regulated Hazardous Chemicals

作者：Zhang, Yan[1,2];Zou, Zhen-Ping[1];Chen, Sheng-Yan[2];Zhou, Ying[1];Ye, Bang-Ce[1,2]

机构：[1]East China Univ Sci & Technol, Inst Engn Biol & Hlth, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, State Key Lab Incubat Base Green Proc Chem Engn, Shihezi 832003, Peoples R China

年份：2023

卷号：95

期号：26

起止页码：9734

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20232814384337);WOS:【SCI-EXPANDED(收录号:WOS:001016735800001)】；

基金：This work was sponsored by the National Natural Science Foundation of China (22134003) and the National Key Research and Development Program of China (2020YFA0908800). We thank the Third Research Institute of the Ministry of Public Security (Shanghai, China) for providing the cocaine used in this study.

语种：英文

外文关键词：DNA sequences - Hazards

摘要：Monitoringand tracing of regulated hazardous chemicals is a publicsecurity issue of global concern. However, accurately recording historicalexposure remains challenging. Here, we designed a Biological Sentinel System (BOSS) for in situ and long-term monitoring ofhazardous chemical exposure using a chemical-induced base-editingsystem that activates antibiotic resistance screening, producing anobvious colorimetric signal. Exposure events can be written into aninheritable genomic DNA sequence, which can be read using gene sequencing.As a proof of concept, we demonstrated the accurate detection of cocaineand 2,4-dinitrotoluene using BOSS under simulated application scenarios.In addition, we integrated alternative biosensors to illustrate themodularity and extensibility of this monitoring platform. This workprovides a promising paradigm for developing engineered microorganismsas an alternative to electronic monitors for regulated hazardous chemicals.