文献类型：期刊文献

英文题名：Multi-scale analysis of nickel ion tolerance mechanism for thermophilic Sulfobacillus thermosulfidooxidans in bioleaching

作者：Chen, Guanglin[1];Shi, Hongjie[1];Ding, Huili[1];Zhang, Xu[1,3];Gu, Tingyue[2];Zhu, Minglong[1];Tan, Wensong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ohio Univ, Inst Sustainable Energy & Environm, Dept Chem & Biomol Engn, Athens, OH USA;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2023

卷号：443

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20224813164033);WOS:【SCI-EXPANDED(收录号:WOS:000882299100005)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21878083) . and the Open Project Funding of the State Key Laboratory of Bioreactor Engineering of China.

语种：英文

外文关键词：Bioleaching; Spent battery; Nickel ion stress; RNA-seq; Transcriptomics

摘要：Bioleaching is intensively investigated for recovering valuable metals such as Li, Co, Ni and Cu. Nickel ion stress threatens the health of microorganisms when Ni2+ starts to accumulate in the leachate during the bioleaching of materials that are rich in Ni, such as spent lithium-ion batteries. The possible mechanisms underlying the response of S. thermosulfidooxidans to nickel ion stress were analyzed using a multi-scale approach. Under the condition of nickel ion stress, high concentrations of nickel ions were immobilized by extracellular polymeric substances, while con-centrations of nickel ions inside the cells remained low. The intracellular adenosine triphosphate (ATP) concen-tration and H+-ATPase activity increased to maintain normal cell growth and metabolic activities. Scavenging abilities of S. thermosulfidooxidans for hydrogen peroxide and superoxide anion were enhanced to reduce oxidative damage induced by nickel ion stress. There were 734 differentially expressed genes identified by RNA-seq under nickel ion stress. Most of them were involved in oxidative phosphorylation, glutathione metabolism and genetic information processing, responsible for intracellular energy utilization, intracellular antioxidant capacity and DNA damage repair, respectively. The results of this study are of major significance for in-depth understanding of the mechanisms of acidophilic microorganisms' resistance to metal ions.